8JLB

Cryo-EM structure of the 145 bp human nucleosome containing H3.2 C110A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15257.8	2	UniProt (Q71DI3) Pfam (PF00125)	Homo sapiens (human)	H3-clustered histone 13,H3-clustered histone 14,H3-clustered histone 15,Histone H3/m,Histone H3/o
2	B, F (B, F)	Histone H4	polymer	106	11676.7	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	133	14447.8	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (D, H)	Histone H2B type 1-J	polymer	129	14217.5	2	UniProt (P06899) Pfam (PF00125)	Homo sapiens (human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I (I)	DNA (145-MER)	polymer	145	44520.4	1		synthetic construct
6	J (J)	DNA (145-MER)	polymer	145	44991.7	1		synthetic construct

Sequence modifications

A, E: 1 - 135 (UniProt: Q71DI3)

PDB	External Database	Details
Ala 110	Cys 111	engineered mutation

B, F: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

C, G: 0 - 129 (UniProt: P04908)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 125 (UniProt: P06899)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	200711.8
All*	Total formula weight	200711.8

*Water molecules are not included.