8JL5

Crystal structure of the Green fluorescent protein SEA227D variant at pH 4.5

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Green fluorescent protein	polymer	238	27002.4	4	UniProt (P42212) Pfam (PF01353)	Aequorea victoria
2	E, F, G, H (A, B, C, D)	water	water		18.0	882	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 238 (UniProt: P42212)

PDB	External Database	Details
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Arg 80	Gln 80	engineered mutation
Asp 147	Ser 147	engineered mutation
Gln 149	Asn 149	engineered mutation
Ala 163	Val 163	engineered mutation
Gly 175	Ser 175	engineered mutation
Phe 202	Ser 202	engineered mutation
Thr 204	Gln 204	engineered mutation
Thr 206	Ala 206	engineered mutation
Asp 227	Ala 227	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	108009.7
All*	Total formula weight	108009.7

*Water molecules are not included.