8JIP
Cryo-EM structure of the GLP-1R/GCGR dual agonist MEDI0382-bound human GLP-1R-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | Glucagon-like peptide 1 receptor | polymer | 440 | 50860.8 | 1 | UniProt (P43220) Pfam (PF02793) Pfam (PF00002) | Homo sapiens (human) | GLP-1 receptor,GLP-1-R,GLP-1R |
| 2 | B (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 361 | 41907.5 | 1 | Pfam (PF00503) UniProt (by SIFTS) (P63092) UniProt (by SIFTS) (P63096) | Homo sapiens (human) | |
| 3 | C (P) | MEDI0382 | polymer | 29 | 3278.4 | 1 | synthetic construct | ||
| 4 | D (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 345 | 37915.5 | 1 | UniProt (P54311) Pfam (PF00400) | Rattus norvegicus (Norway rat) | Transducin beta chain 1 |
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (cattle) | G gamma-I |
| 6 | F (N) | Nanobody 35 | polymer | 140 | 15343.0 | 1 | Escherichia coli | ||
| 7 | G (P) | N-hexadecanoyl-L-glutamic acid | non-polymer | 385.5 | 1 | Chemie (D6M) |
Sequence modifications
B: 2 - 340 (UniProt: P54311)
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 157035.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 385.5 | |
| All* | Total formula weight | 157420.7 |
