8JHY
Cryo-EM structure of compound 9n bound ketone body receptor HCAR2-Gi signaling complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 356 | 39141.8 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | Transducin beta chain 1 |
| 2 | B (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 3 | C (D) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 4 | D (S) | scFv16 | polymer | 266 | 28636.8 | 1 | Mus musculus | ||
| 5 | E (A) | Hydroxycarboxylic acid receptor 2 | polymer | 397 | 46034.1 | 1 | UniProt (Q8TDS4) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 109A,G-protein coupled receptor HM74A,Niacin receptor 1,Nicotinic acid receptor |
| 6 | F (A) | 7-methyl-N-[(2R)-1-phenoxypropan-2-yl]-3-(4-propan-2-ylphenyl)pyrazolo[1,5-a]pyrimidine-6-carboxamide | non-polymer | 428.5 | 1 | Chemie (IX8) | |||
| 7 | G (A) | CHOLESTEROL | non-polymer | 386.7 | 1 | Chemie (CLR) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
D: 1 - 354 (UniProt: P63096)
A: 1 - 363 (UniProt: Q8TDS4)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| Leu -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Val -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Gln -5 | - | expression tag |
| Gly -4 | - | expression tag |
| Pro -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -33 | - | initiating methionine |
| Lys -32 | - | expression tag |
| Thr -31 | - | expression tag |
| Ile -30 | - | expression tag |
| Ile -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Leu -27 | - | expression tag |
| Ser -26 | - | expression tag |
| Tyr -25 | - | expression tag |
| Ile -24 | - | expression tag |
| Phe -23 | - | expression tag |
| Cys -22 | - | expression tag |
| Leu -21 | - | expression tag |
| Val -20 | - | expression tag |
| Phe -19 | - | expression tag |
| Ala -18 | - | expression tag |
| Asp -17 | - | expression tag |
| Tyr -16 | - | expression tag |
| Lys -15 | - | expression tag |
| Asp -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Asp -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Ala -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 162087.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 815.2 | |
| All* | Total formula weight | 162903.0 |
