8JHB

FZD6 Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas	polymer	270	31117.2	1	UniProt (Q5JWF2) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein,Extra large alphas protein,XLalphas
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	74	8417.8	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nb35	polymer	135	14845.5	1		Homo sapiens (human)
5	E (R)	Frizzled-6	polymer	537	61912.4	1	UniProt (O60353) Pfam (PF01392) Pfam (PF01534)	Homo sapiens (human)	Fz-6,hFz6

Sequence modifications

A: 7 - 59 (UniProt: Q5JWF2)

PDB	External Database	Details
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag
Ser 2	-	expression tag
Lys 3	-	expression tag
Thr 4	-	expression tag
Glu 5	-	expression tag
Asp 6	-	expression tag
Asp 44	Gly 692	engineered mutation
Asn 45	Glu 693	engineered mutation

A: 204 - 253 (UniProt: Q5JWF2)

PDB	External Database	Details
Gly 60	-	linker
Gly 61	-	linker
Ser 62	-	linker
Gly 63	-	linker
Gly 64	-	linker
Ser 65	-	linker
Gly 66	-	linker
Gly 67	-	linker
Asp 249	Ala 892	engineered mutation
Asp 252	Ser 895	engineered mutation

A: 264 - 394 (UniProt: Q5JWF2)

PDB	External Database	Details
Gly 254	-	linker
Ser 255	-	linker
Gly 256	-	linker
Gly 257	-	linker
Ser 258	-	linker
Gly 259	-	linker
Ala 260	-	linker
Gly 261	-	linker
Ser 262	-	linker
Ala 263	-	linker
Ala 372	Ile 1015	engineered mutation
Ile 375	Val 1018	engineered mutation

C: 29 - 95 (UniProt: P59768)

PDB	External Database	Details
Met 22	-	initiating methionine
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag
His 27	-	expression tag
His 28	-	expression tag

R: 19 - 525 (UniProt: O60353)

PDB	External Database	Details
Met -11	-	initiating methionine
Lys -10	-	expression tag
Thr -9	-	expression tag
Ile -8	-	expression tag
Ile -7	-	expression tag
Ala -6	-	expression tag
Leu -5	-	expression tag
Ser -4	-	expression tag
Tyr -3	-	expression tag
Ile -2	-	expression tag
Phe -1	-	expression tag
Cys 0	-	expression tag
Leu 1	-	expression tag
Val 2	-	expression tag
Phe 3	-	expression tag
Ala 4	-	expression tag
Asp 5	-	expression tag
Tyr 6	-	expression tag
Lys 7	-	expression tag
Asp 8	-	expression tag
Asp 9	-	expression tag
Asp 10	-	expression tag
Asp 11	-	expression tag
Lys 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	153709.8
All*	Total formula weight	153709.8

*Water molecules are not included.