8JH2

RNA polymerase II elongation complex bound with Elf1, Spt4/5 and foreign DNA, stalled at SHL(-1) of the nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	0	DNA (40-MER)	polymer	40	12306.0	1		synthetic construct
2	1	DNA (40-MER)	polymer	40	12316.8	1		synthetic construct
3	A	DNA-directed RNA polymerase subunit	polymer	1743	194107.4	1	UniProt (C4R4Y0) Pfam (PF04997) Pfam (PF05001) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) In PDB	Komagataella phaffii
4	B	DNA-directed RNA polymerase subunit beta	polymer	1227	139746.1	1	UniProt (C4QZQ7) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) In PDB	Komagataella phaffii
5	C	RNA polymerase II third largest subunit B44, part of central core	polymer	304	34216.3	1	UniProt (C4R7L2) Pfam (PF01193) Pfam (PF01000) In PDB	Komagataella phaffii
6	D	RNA polymerase II subunit B32	polymer	186	20623.0	1	UniProt (C4R2U9) Pfam (PF03874) In PDB	Komagataella phaffii
7	E	RNA polymerase subunit ABC27	polymer	214	24962.7	1	UniProt (C4R3P8) Pfam (PF03871) Pfam (PF01191) In PDB	Komagataella phaffii	RNA polymerases I, II, and III
8	F	RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III	polymer	155	17803.6	1	UniProt (C4R1V1) Pfam (PF01192) In PDB	Komagataella phaffii
9	G	RNA polymerase II subunit	polymer	171	18802.6	1	UniProt (C4R9A1) Pfam (PF03876) Pfam (PF00575) In PDB	Komagataella phaffii
10	H	DNA-directed RNA polymerases I, II, and III subunit RPABC3	polymer	145	16249.2	1	UniProt (C4R273) Pfam (PF03870) In PDB	Komagataella phaffii
11	I	DNA-directed RNA polymerase subunit	polymer	115	13612.3	1	UniProt (F2QPE6) Pfam (PF02150) Pfam (PF01096) In PDB	Komagataella phaffii
12	J	RNA polymerase subunit ABC10-beta, common to RNA polymerases I, II, and III	polymer	72	8554.1	1	UniProt (C4R009) Pfam (PF01194) In PDB	Komagataella phaffii
13	K	RNA polymerase II subunit B12.5	polymer	118	13832.9	1	UniProt (C4R3Z5) Pfam (PF13656) In PDB	Komagataella phaffii
14	L	RNA polymerase subunit ABC10-alpha	polymer	72	7862.0	1	UniProt (F2QMI1) Pfam (PF03604) In PDB	Komagataella phaffii
15	M	Transcription elongation factor 1 homolog	polymer	110	12395.7	1	UniProt (C4QZ45) Pfam (PF05129) In PDB	Komagataella phaffii
16	N	DNA (218-MER)	polymer	228	70594.7	1		synthetic construct
17	P	RNA (5'-R(P*CP*CP*UP*GP*GP*UP*GP*UP*CP*UP*UP*GP*GP*GP*UP*G)-3')	polymer	16	5124.0	1		synthetic construct
18	T	DNA (218-MER)	polymer	228	70193.9	1		synthetic construct
19	V	Transcription elongation factor SPT4	polymer	114	12627.3	1	UniProt (F2QTA2) Pfam (PF06093) UniProt (by SIFTS) (C4R0E6) In PDB	Komagataella phaffii
20	W	Transcription elongation factor SPT5	polymer	908	101248.2	1	UniProt (C4R370) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) Pfam (PF12815) In PDB	Komagataella phaffii
21	a, e	Histone H3.3	polymer	136	15361.0	2	UniProt (P84243) Pfam (PF00125) In PDB	Homo sapiens (human)
22	b, f	Histone H4	polymer	103	11394.4	2	UniProt (P62805) Pfam (PF15511) In PDB	Homo sapiens (human)
23	c, g	Histone H2A type 1-B/E	polymer	130	14165.6	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211) In PDB	Homo sapiens (human)
24	d, h	Histone H2B type 1-J	polymer	126	13935.2	2	UniProt (P06899) Pfam (PF00125) In PDB	Homo sapiens (human)	Histone H2B.1,Histone H2B.r,H2B/r
25	A, B, C, I, J...	ZINC ION	non-polymer		65.4	10	Chemie (ZN)
26	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	28
	Total formula weight	916891.3
Non-Polymers*	Number of molecules	11
	Total formula weight	678.4
All*	Total formula weight	917569.7

*Water molecules are not included.