8JGF

CryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein Gq	polymer	361	41724.4	1		Homo sapiens (human)
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	352	38561.2	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
3	C (E)	Single-Chain Fragment Variable 16	polymer	285	30363.0	1		Homo sapiens (human)
4	D (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	58	6375.3	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (L)	BAM8-22	polymer	15	1974.2	1	UniProt (by SIFTS) (P01210)	Homo sapiens
6	F (R)	Mas-related G-protein coupled receptor member X1	polymer	319	35906.5	1	UniProt (Q96LB2) Pfam (PF00001)	Homo sapiens (human)	Sensory neuron-specific G-protein coupled receptor 3/4

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Pro 1	-	expression tag
Cys 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
Ser 5	-	expression tag
Gly 6	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	154904.6
All*	Total formula weight	154904.6

*Water molecules are not included.