8JGC

Cryo-EM structure of Mi3 fused with LOV2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	LOV domain-containing protein,2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase	polymer	378	41424.5	1	UniProt (A0A453KFI0) UniProt (Q9WXS1) Pfam (PF13426) Pfam (PF01081) In PDB	Aegilops tauschii subsp. strangulata (Goatgrass)

Sequence modifications

A: -157 - -18 (UniProt: A0A453KFI0)

PDB	External Database	Details
Met -163	-	initiating methionine
Gly -162	-	expression tag
Ser -161	-	expression tag
Ser -160	-	expression tag
Glu -159	-	expression tag
Phe -158	-	expression tag
Ala -100	Asp 99	conflict
Val -68	Leu 131	conflict
Tyr -59	Gln 140	conflict
Arg -42	His 157	conflict
Leu -41	Val 158	conflict
His -40	Arg 159	conflict
Gly -39	Asp 160	conflict
Arg -35	Lys 164	conflict
Ala -33	Gly 166	conflict
Cys -31	Met 168	conflict
Phe -24	Glu 175	conflict
Gln -23	Asn 176	conflict
Ala -21	Asp 178	conflict
Asn -17	-	linker
Asp -16	-	linker
Glu -15	-	linker
Asn -14	-	linker
Tyr -13	-	linker
Phe -12	-	linker
Gly -11	-	linker
Gly -10	-	linker
Ser -9	-	linker
Gly -8	-	linker
Gly -7	-	linker
Ser -6	-	linker
Gly -5	-	linker
Gly -4	-	linker
Ser -3	-	linker
Gly -2	-	linker
Gly -1	-	linker
Ser 0	-	linker

A: 1 - 205 (UniProt: Q9WXS1)

PDB	External Database	Details
Lys 26	Glu 26	conflict
Leu 33	Glu 33	conflict
Met 61	Lys 61	conflict
Ala 76	Cys 76	conflict
Ala 100	Cys 100	conflict
Val 187	Asp 187	conflict
Ala 190	Arg 190	conflict
Gly 206	-	expression tag
Ser 207	-	expression tag
Gly 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	41424.5
All*	Total formula weight	41424.5

*Water molecules are not included.