8JD2

Cryo-EM structure of G protein-free mGlu2-mGlu3 heterodimer in Acc state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	Metabotropic glutamate receptor 2,Peptidyl-prolyl cis-trans isomerase FKBP1A	polymer	993	109398.9	1	UniProt (Q14416) UniProt (P62942) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) Pfam (PF00254)	Homo sapiens (human)	mGluR2,PPIase FKBP1A,12 kDa FK506-binding protein,12 kDa FKBP,FKBP-12,Calstabin-1,FK506-binding protein 1A,FKBP-1A,Immunophilin FKBP12,Rotamase
2	B (3)	Metabotropic glutamate receptor 3,Serine/threonine-protein kinase mTOR	polymer	993	112713.0	1	UniProt (Q14832) UniProt (A0A8V8TRG9) Pfam (PF01094) Pfam (PF07562) Pfam (PF00003) Pfam (PF08771)	Homo sapiens (human)	mGluR3
3	C, E (2, 3)	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
4	D, F (2, 3)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

2: 19 - 872 (UniProt: Q14416)

PDB	External Database	Details
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Gly 16	-	expression tag
Ala 17	-	expression tag
Pro 18	-	expression tag
Leu 873	-	linker
Glu 874	-	linker
Val 875	-	linker
Leu 876	-	linker
Phe 877	-	linker
Gln 878	-	linker
Gly 879	-	linker
Pro 880	-	linker

2: 881 - 987 (UniProt: P62942)

PDB	External Database	Details
Phe 988	-	expression tag
Ala 989	-	expression tag
Ala 990	-	expression tag
Ala 991	-	expression tag
His 992	-	expression tag
His 993	-	expression tag
His 994	-	expression tag
His 995	-	expression tag
His 996	-	expression tag
His 997	-	expression tag
His 998	-	expression tag
His 999	-	expression tag
His 1000	-	expression tag
His 1001	-	expression tag

3: 23 - 879 (UniProt: Q14832)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
Gly 0	-	expression tag
Ala 1	-	expression tag
Pro 2	-	expression tag
Trp 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
Pro 6	-	expression tag
Gln 7	-	expression tag
Phe 8	-	expression tag
Glu 9	-	expression tag
Lys 10	-	expression tag
Gly 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
Trp 15	-	expression tag
Ser 16	-	expression tag
His 17	-	expression tag
Pro 18	-	expression tag
Gln 19	-	expression tag
Phe 20	-	expression tag
Glu 21	-	expression tag
Lys 22	-	expression tag
Leu 880	-	linker
Glu 881	-	linker
Val 882	-	linker
Leu 883	-	linker
Phe 884	-	linker
Gln 885	-	linker
Gly 886	-	linker
Pro 887	-	linker

3: 888 - 982 (UniProt: A0A8V8TRG9)

PDB	External Database	Details
Glu 983	-	expression tag
Phe 984	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	222111.9
Non-Polymers*	Number of molecules	4
	Total formula weight	736.7
All*	Total formula weight	222848.5

*Water molecules are not included.