8J9N

Gq bound FZD1 in ligand-free state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Frizzled-1	polymer	579	64015.7	1	UniProt (Q9UP38) Pfam (PF01392) Pfam (PF01534)	Homo sapiens (human)	Fz-1,hFz1,FzE1
2	B (C)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short,Guanine nucleotide-binding protein G(q) subunit alpha	polymer	249	28820.5	1	UniProt (P63092) UniProt (P50148) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein,Guanine nucleotide-binding protein alpha-q
3	C (D)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
4	D (E)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7845.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
5	E (N)	nanobody Nb35	polymer	149	16054.2	1		Vicugna pacos

Sequence modifications

C: 5 - 27 (UniProt: P63092)
C: 28 - 37 (UniProt: P50148)
C: 38 - 195 (UniProt: P63092)

PDB	External Database	Details
Asp 49	Gly 49	engineered mutation
Asn 50	Glu 50	engineered mutation

C: 204 - 379 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	engineered mutation
Asp 252	Ser 252	engineered mutation
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Ala 372	Ile 372	engineered mutation
Ile 375	Val 375	engineered mutation

C: 380 - 394 (UniProt: P50148)
E: 25 - 95 (UniProt: P59768)

PDB	External Database	Details
Ser 92	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	154152.4
All*	Total formula weight	154152.4

*Water molecules are not included.