8J90

Cryo-EM structure of DDM1-nucleosome complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.1	polymer	139	15583.2	2	UniProt (P59226) Pfam (PF00125)	Arabidopsis thaliana (thale cress)
2	B, F (B, F)	Histone H4	polymer	106	11718.7	2	UniProt (P59259) Pfam (PF15511)	Arabidopsis thaliana (thale cress)
3	C, G (C, G)	HTA6	polymer	153	16280.2	2	UniProt (Q9FJE8) Pfam (PF00125) Pfam (PF16211)	Arabidopsis thaliana (thale cress)	HTA6
4	D, H (D, H)	HTB9	polymer	153	16756.7	2	UniProt (O23629) Pfam (PF00125)	Arabidopsis thaliana (thale cress)	H2BAt,HTB9
5	I (I)	DNA (169-MER)	polymer	169	51922.1	1		synthetic construct
6	J (J)	DNA (169-MER)	polymer	169	52424.4	1		synthetic construct
7	K (K)	ATP-dependent DNA helicase DDM1	polymer	767	87050.1	1	UniProt (Q9XFH4) Pfam (PF00176) Pfam (PF00271)	Arabidopsis thaliana (thale cress)	AtDDM1

Sequence modifications

A, E: 0 - 135 (UniProt: P59226)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

B, F: 0 - 102 (UniProt: P59259)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

C, G: 0 - 149 (UniProt: Q9FJE8)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 149 (UniProt: O23629)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag

K: 1 - 764 (UniProt: Q9XFH4)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	11
	Total formula weight	312074.4
All*	Total formula weight	312074.4

*Water molecules are not included.