8J8G
Monkeypox virus DNA replication holoenzyme F8, A22 and E4 in complex with a DNA duplex and cidofovir diphosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 1029 | 120041.2 | 1 | UniProt (Q5IXW8) Pfam (PF08452) Pfam (PF03104) Pfam (PF08408) Pfam (PF00136) | Monkeypox virus | |
| 2 | B (B) | E4R | polymer | 241 | 27883.7 | 1 | UniProt (Q5IXS4) | Monkeypox virus | |
| 3 | C (C) | DNA polymerase processivity factor component A20 | polymer | 426 | 49203.9 | 1 | UniProt (Q5IXP2) Pfam (PF05941) | Monkeypox virus | |
| 4 | D (P) | DNA (5'-D(P*AP*GP*CP*TP*GP*CP*TP*AP*TP*GP*AP*GP*AP*TP*TP*AP*AP*GP*TP*TP*AP*T)-3') | polymer | 24 | 7438.8 | 1 | DNA molecule | ||
| 5 | E (T) | DNA (5'-D(P*GP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*GP*AP*TP*AP*AP*CP*TP*TP*AP*AP*TP*CP*TP*CP*AP*CP*AP*TP*AP*GP*CP*AP*GP*CP*T)-3') | polymer | 38 | 11605.5 | 1 | DNA molecule | ||
| 6 | F (A) | [(2S)-1-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3-oxidanyl-propan-2-yl]oxymethyl-[oxidanyl(phosphonooxy)phosphoryl]oxy-phosphinic acid | non-polymer | 439.1 | 1 | Chemie (TXJ) | |||
| 7 | G, H, I (A) | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) |
Sequence modifications
A: 1 - 1006 (UniProt: Q5IXW8)
B: 1 - 218 (UniProt: Q5IXS4)
| PDB | External Database | Details |
|---|---|---|
| Met -22 | - | initiating methionine |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Tyr -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Ile -10 | - | expression tag |
| Pro -9 | - | expression tag |
| Thr -8 | - | expression tag |
| Thr -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Asn -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Phe -2 | - | expression tag |
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Phe 108 | Leu 108 | engineered mutation |
| Leu 411 | Trp 411 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -22 | - | initiating methionine |
| His -21 | - | expression tag |
| His -20 | - | expression tag |
| His -19 | - | expression tag |
| His -18 | - | expression tag |
| His -17 | - | expression tag |
| His -16 | - | expression tag |
| Asp -15 | - | expression tag |
| Tyr -14 | - | expression tag |
| Asp -13 | - | expression tag |
| Ile -12 | - | expression tag |
| Pro -11 | - | expression tag |
| Thr -10 | - | expression tag |
| Thr -9 | - | expression tag |
| Glu -8 | - | expression tag |
| Asn -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Tyr -5 | - | expression tag |
| Phe -4 | - | expression tag |
| Gln -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 216173.1 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 559.4 | |
| All* | Total formula weight | 216732.5 |
