8J7R
Cryo-EM structure of the J-K-St region of EMCV IRES in complex with eIF4G-HEAT1 and eIF4A (J-K-St/eIF4G focused)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | Eukaryotic translation initiation factor 4 gamma 1 | polymer | 270 | 31786.8 | 1 | UniProt (Q04637) Pfam (PF02854) | Homo sapiens (human) | eIF-4-gamma 1,eIF-4G 1,eIF-4G1,p220 |
| 2 | B (C) | IRES RNA (J-K-St) | polymer | 108 | 34838.6 | 1 | GenBank (NC_001479.1) | Encephalomyocarditis virus | |
| 3 | C, D, E (C) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 4 | F (C) | water | water | 18.0 | 1 | Chemie (HOH) |
Sequence modifications
B: 746 - 992 (UniProt: Q04637)
| PDB | External Database | Details |
|---|---|---|
| Met 723 | - | initiating methionine |
| Asn 724 | - | expression tag |
| His 725 | - | expression tag |
| Lys 726 | - | expression tag |
| Val 727 | - | expression tag |
| His 728 | - | expression tag |
| His 729 | - | expression tag |
| His 730 | - | expression tag |
| His 731 | - | expression tag |
| His 732 | - | expression tag |
| His 733 | - | expression tag |
| Ile 734 | - | expression tag |
| Glu 735 | - | expression tag |
| Gly 736 | - | expression tag |
| Arg 737 | - | expression tag |
| His 738 | - | expression tag |
| Met 739 | - | expression tag |
| Glu 740 | - | expression tag |
| Leu 741 | - | expression tag |
| Gly 742 | - | expression tag |
| Thr 743 | - | expression tag |
| Leu 744 | - | expression tag |
| Glu 745 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 66625.4 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 72.9 | |
| All* | Total formula weight | 66698.3 |
