8J7R

Cryo-EM structure of the J-K-St region of EMCV IRES in complex with eIF4G-HEAT1 and eIF4A (J-K-St/eIF4G focused)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	Eukaryotic translation initiation factor 4 gamma 1	polymer	270	31786.8	1	UniProt (Q04637) Pfam (PF02854) In PDB	Homo sapiens (human)	eIF-4-gamma 1,eIF-4G 1,eIF-4G1,p220
2	C	IRES RNA (J-K-St)	polymer	108	34838.6	1	GenBank (NC_001479.1)	Encephalomyocarditis virus
3	C	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
4		water	water		18.0	1	Chemie (HOH)

Sequence modifications

B: 746 - 992 (UniProt: Q04637)

PDB	External Database	Details
Met 723	-	initiating methionine
Asn 724	-	expression tag
His 725	-	expression tag
Lys 726	-	expression tag
Val 727	-	expression tag
His 728	-	expression tag
His 729	-	expression tag
His 730	-	expression tag
His 731	-	expression tag
His 732	-	expression tag
His 733	-	expression tag
Ile 734	-	expression tag
Glu 735	-	expression tag
Gly 736	-	expression tag
Arg 737	-	expression tag
His 738	-	expression tag
Met 739	-	expression tag
Glu 740	-	expression tag
Leu 741	-	expression tag
Gly 742	-	expression tag
Thr 743	-	expression tag
Leu 744	-	expression tag
Glu 745	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	66625.4
Non-Polymers*	Number of molecules	3
	Total formula weight	72.9
All*	Total formula weight	66698.3

*Water molecules are not included.