8J5T

Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the catalytic intermediate state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Uncharacterized protein Rv1280c	polymer	599	64501.1	1	UniProt (P9WGU5) Pfam (PF00496)	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
2	B (B)	Putative peptide transport permease protein Rv1283c	polymer	325	35120.1	1	UniProt (P9WFZ7) Pfam (PF19300) Pfam (PF00528)	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Oligopeptide-transport integral membrane protein OppB
3	C (C)	Putative peptide transport permease protein Rv1282c	polymer	291	31402.1	1	UniProt (P9WFZ9) Pfam (PF12911) Pfam (PF00528)	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Oligopeptide-transport integral membrane protein OppC
4	D (D)	Uncharacterized ABC transporter ATP-binding protein Rv1281c	polymer	612	65342.8	1	UniProt (P9WQJ5) Pfam (PF00005) Pfam (PF08352)	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Oligopeptide-transport ATP-binding protein OppD
5	E, G (D)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
6	F, H (D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	I (D)	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)

Sequence modifications

A: 1 - 591 (UniProt: P9WGU5)

PDB	External Database	Details
Val 1	Met 1	conflict
Asp 592	-	expression tag
Tyr 593	-	expression tag
Lys 594	-	expression tag
Asp 595	-	expression tag
Asp 596	-	expression tag
Asp 597	-	expression tag
Asp 598	-	expression tag
Lys 599	-	expression tag

D: 1 - 612 (UniProt: P9WQJ5)

PDB	External Database	Details
Asp 180	Glu 180	engineered mutation
Asp 525	Glu 525	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	196366.2
Non-Polymers*	Number of molecules	5
	Total formula weight	1414.6
All*	Total formula weight	197780.8

*Water molecules are not included.