8J5S

Cryo-EM structure of Mycobacterium tuberculosis OppABCD in the pre-catalytic intermediate state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Uncharacterized protein Rv1280c	polymer	599	64501.1	1	UniProt (P9WGU5) Pfam (PF00496) In PDB	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
2	B	Putative peptide transport permease protein Rv1283c	polymer	325	35120.1	1	UniProt (P9WFZ7) Pfam (PF19300) Pfam (PF00528) In PDB	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
3	C	Putative peptide transport permease protein Rv1282c	polymer	291	31402.1	1	UniProt (P9WFZ9) Pfam (PF12911) Pfam (PF00528) In PDB	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
4	D	Uncharacterized ABC transporter ATP-binding protein Rv1281c	polymer	612	65342.8	1	UniProt (P9WQJ5) Pfam (PF00005) Pfam (PF08352) In PDB	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
5	E	Endogenous oligopeptide	polymer	8	698.9	1		Mycolicibacterium smegmatis MC2 155
6	D	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	2	Chemie (ANP)
7	D	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	D	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)

Sequence modifications

A: 1 - 591 (UniProt: P9WGU5)

PDB	External Database	Details
Val 1	Met 1	conflict
Asp 592	-	expression tag
Tyr 593	-	expression tag
Lys 594	-	expression tag
Asp 595	-	expression tag
Asp 596	-	expression tag
Asp 597	-	expression tag
Asp 598	-	expression tag
Lys 599	-	expression tag

D: 1 - 612 (UniProt: P9WQJ5)

PDB	External Database	Details
Asp 180	Glu 180	engineered mutation
Asp 525	Glu 525	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	197065.0
Non-Polymers*	Number of molecules	5
	Total formula weight	1412.6
All*	Total formula weight	198477.7

*Water molecules are not included.