8J5N

Crystal structure of a PETase variant V20 from Ideonella sakaiensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	Poly(ethylene terephthalate) hydrolase	polymer	298	31375.0	2	UniProt (A0A0K8P6T7) Pfam (PF01738)	Ideonella sakaiensis	PET hydrolase,PETase,PET-digesting enzyme
2	C, D (A, D)	water	water		18.0	209	Chemie (HOH)

Sequence modifications

A, D: 1 - 290 (UniProt: A0A0K8P6T7)

PDB	External Database	Details
Glu 14	Val 14	engineered mutation
Pro 18	Leu 18	engineered mutation
Gln 53	Arg 53	engineered mutation
Leu 84	Val 84	engineered mutation
His 186	Asp 186	engineered mutation
Ile 201	Phe 201	engineered mutation
Tyr 229	Phe 229	engineered mutation
Lys 233	Asn 233	engineered mutation
Glu 280	Arg 280	engineered mutation
Arg 283	Asp 283	engineered mutation
Leu 291	-	expression tag
Glu 292	-	expression tag
His 293	-	expression tag
His 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	62749.9
All*	Total formula weight	62749.9

*Water molecules are not included.