8J5M

Structure of GH1 Br2 beta-glucosidase E350G mutant from bovine rumen metagenome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, D, C)	Beta-glucosidase	polymer	465	53016.7	4	UniProt (A0A1S5SJM8)	uncultured bacterium
2	E, LA, N, O, TA... (A, C, B, D)	ACETATE ION	non-polymer		59.0	9	Chemie (ACT)
3	AA, BA, CA, DA, EA... (D, A, C, B)	SULFATE ION	non-polymer		96.1	33	Chemie (SO4)
4	UA, VA, WA, XA (A, B, D, C)	water	water		18.0	2107	Chemie (HOH)

Sequence modifications

A, B, D, C: 1 - 445 (UniProt: A0A1S5SJM8)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 350	Glu 350	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	212066.7
Non-Polymers*	Number of molecules	42
	Total formula weight	3701.5
All*	Total formula weight	215768.2

*Water molecules are not included.