8J5L

Structure of GH1 Br2 beta-glucosidase E163Q mutant from bovine rumen metagenome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Beta-glucosidase	polymer	465	53087.8	4	UniProt (A0A1S5SJM8) Pfam (PF00232)	uncultured bacterium
2	E, K, P, S (A, B, C, D)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
3	F, G, H, I, J... (A, B, C, D)	SULFATE ION	non-polymer		96.1	15	Chemie (SO4)
4	AA, X, Y, Z (D, A, B, C)	water	water		18.0	338	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 445 (UniProt: A0A1S5SJM8)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gln 163	Glu 163	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	212351.0
Non-Polymers*	Number of molecules	19
	Total formula weight	1809.3
All*	Total formula weight	214160.4

*Water molecules are not included.