8J5D

Cryo-EM structure of starch degradation complex of BAM1-LSF1-MDH

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta-amylase 1, chloroplastic	polymer	499	56112.0	1	UniProt (Q9LIR6) Pfam (PF01373) In PDB	Arabidopsis thaliana (thale cress)	BAM1,1,4-alpha-D-glucan maltohydrolase,Beta-amylase 7,Thioredoxin-regulated beta-amylase,TR-BAMY
2	B, C	Malate dehydrogenase, chloroplastic	polymer	323	34120.1	2	UniProt (Q9SN86) Pfam (PF00056) Pfam (PF02866) In PDB	Arabidopsis thaliana (thale cress)	Chloroplastic malate dehydrogenase,Chloroplastic MDH,cpNAD-MDH,Plastidic NAD-dependent malate dehydrogenase,pNAD-MDH
3	D	Phosphoglucan phosphatase LSF1, chloroplastic	polymer	529	59231.2	1	UniProt (F4J117) Pfam (PF00782) Pfam (PF16561) In PDB	Arabidopsis thaliana (thale cress)	Phosphoglucan phosphatase like sex Four1,Protein LIKE SEX4 1

Sequence modifications

A: 105 - 575 (UniProt: Q9LIR6)

PDB	External Database	Details
Met 77	-	initiating methionine
Gly 78	-	expression tag
Ser 79	-	expression tag
Ser 80	-	expression tag
His 81	-	expression tag
His 82	-	expression tag
His 83	-	expression tag
His 84	-	expression tag
His 85	-	expression tag
His 86	-	expression tag
Ser 87	-	expression tag
Ser 88	-	expression tag
Gly 89	-	expression tag
Leu 90	-	expression tag
Val 91	-	expression tag
Pro 92	-	expression tag
Arg 93	-	expression tag
Gly 94	-	expression tag
Ser 95	-	expression tag
His 96	-	expression tag
Ser 97	-	expression tag
Asp 98	-	expression tag
Glu 99	-	expression tag
Val 100	-	expression tag
Asp 101	-	expression tag
Ala 102	-	expression tag
His 103	-	expression tag
Met 104	-	expression tag

D: 71 - 591 (UniProt: F4J117)

PDB	External Database	Details
Leu 592	-	expression tag
Glu 593	-	expression tag
His 594	-	expression tag
His 595	-	expression tag
His 596	-	expression tag
His 597	-	expression tag
His 598	-	expression tag
His 599	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	183583.5
All*	Total formula weight	183583.5

*Water molecules are not included.