8J52

Crystal structure of Flavihumibacter petaseus GH31 alpha-galactosidase mutant D304A in complex with alpha-1,4-galactobiose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	GH31 alpha-galactosidase	polymer	541	60929.3	2	UniProt (A0A0E9MUN5) Pfam (PF01055) Pfam (PF21365) In PDB	Flavihumibacter petaseus NBRC 106054
2	A, B	alpha-D-galactopyranose	non-polymer		180.2	2	Chemie (GLA)
3	A, B	beta-D-galactopyranose	non-polymer		180.2	2	Chemie (GAL)
4	A, B	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
5	A, B	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6		water	water		18.0	794	Chemie (HOH)

Sequence modifications

A, B: 29 - 546 (UniProt: A0A0E9MUN5)

PDB	External Database	Details
Met 6	-	initiating methionine
Gly 7	-	expression tag
Ser 8	-	expression tag
Ser 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
His 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
Ser 16	-	expression tag
Ser 17	-	expression tag
Gly 18	-	expression tag
Leu 19	-	expression tag
Val 20	-	expression tag
Pro 21	-	expression tag
Arg 22	-	expression tag
Gly 23	-	expression tag
Ser 24	-	expression tag
His 25	-	expression tag
Met 26	-	expression tag
Ala 27	-	expression tag
Ser 28	-	expression tag
Ala 304	Asp 304	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	121858.7
Non-Polymers*	Number of molecules	17
	Total formula weight	1474.3
All*	Total formula weight	123332.9

*Water molecules are not included.