8J45
Crystal structure of a Pichia pastoris-expressed IsPETase variant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Poly(ethylene terephthalate) hydrolase | polymer | 263 | 27592.6 | 1 | UniProt (A0A0K8P6T7) Pfam (PF12740) | Ideonella sakaiensis | PET hydrolase,PETase,PET-digesting enzyme |
| 2 | B, C, D, E (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
| 3 | F (A) | water | water | 18.0 | 348 | Chemie (HOH) |
Sequence modifications
A: 30 - 290 (UniProt: A0A0K8P6T7)
| PDB | External Database | Details |
|---|---|---|
| Glu 28 | - | expression tag |
| Phe 29 | - | expression tag |
| Glu 121 | Ser 121 | engineered mutation |
| His 186 | Asp 186 | engineered mutation |
| Gln 224 | Arg 224 | engineered mutation |
| Lys 233 | Asn 233 | engineered mutation |
| Ala 280 | Arg 280 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 27592.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 884.8 | |
| All* | Total formula weight | 28477.5 |
