8J30

Crystal structure of ApNGT with Q469A and M218A mutations in complex with UDP-GLC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	UDP-glucose:protein N-beta-glucosyltransferase	polymer	628	71532.2	2	UniProt (A3N2T3) Pfam (PF18071) Pfam (PF18254)	Actinobacillus pleuropneumoniae serovar 5b str. L20	UDP-Glc:protein N-glucosyltransferase,HMW1C-like protein,N-glycosyltransferase,NGT
2	C (A)	URIDINE-5'-DIPHOSPHATE-GLUCOSE	non-polymer		566.3	1	Chemie (UPG)
3	D (B)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	1	Chemie (UDP)

Sequence modifications

A, B: 1 - 620 (UniProt: A3N2T3)

PDB	External Database	Details
Ala 218	Met 218	engineered mutation
Ala 469	Gln 469	engineered mutation
Leu 621	-	expression tag
Glu 622	-	expression tag
His 623	-	expression tag
His 624	-	expression tag
His 625	-	expression tag
His 626	-	expression tag
His 627	-	expression tag
His 628	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	143064.5
Non-Polymers*	Number of molecules	2
	Total formula weight	970.5
All*	Total formula weight	144034.9

*Water molecules are not included.