8J20
Cryo-EM structure of FFAR3 bound with valeric acid and AR420626
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 377 | 41055.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 2 | B (B) | scFV16 | polymer | 297 | 31870.6 | 1 | Homo sapiens (human) | ||
| 3 | C (C) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40415.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 4 | D (D) | Free fatty acid receptor 3 | polymer | 314 | 35432.6 | 1 | UniProt (O14843) Pfam (PF00001) | Homo sapiens (human) | G-protein coupled receptor 41 |
| 5 | E (E) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 6 | F (D) | (4R)-N-[2,5-bis(chloranyl)phenyl]-4-(furan-2-yl)-2-methyl-5-oxidanylidene-4,6,7,8-tetrahydro-1H-quinoline-3-carboxamide | non-polymer | 417.3 | 1 | Chemie (9T4) | |||
| 7 | G (D) | PENTANOIC ACID | non-polymer | 102.1 | 1 | Chemie (LEA) |
Sequence modifications
A: 13 - 351 (UniProt: P62873)
D: 1 - 314 (UniProt: O14843)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| His 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| Gly 8 | - | expression tag |
| Ser 9 | - | expression tag |
| Leu 10 | - | expression tag |
| Leu 11 | - | expression tag |
| Gln 12 | - | expression tag |
| Gly 352 | - | expression tag |
| Ser 353 | - | expression tag |
| Ser 354 | - | expression tag |
| Gly 355 | - | expression tag |
| Gly 356 | - | expression tag |
| Gly 357 | - | expression tag |
| Gly 358 | - | expression tag |
| Ser 359 | - | expression tag |
| Gly 360 | - | expression tag |
| Gly 361 | - | expression tag |
| Gly 362 | - | expression tag |
| Gly 363 | - | expression tag |
| Ser 364 | - | expression tag |
| Ser 365 | - | expression tag |
| Gly 366 | - | expression tag |
| Val 367 | - | expression tag |
| Ser 368 | - | expression tag |
| Gly 369 | - | expression tag |
| Trp 370 | - | expression tag |
| Arg 371 | - | expression tag |
| Leu 372 | - | expression tag |
| Phe 373 | - | expression tag |
| Lys 374 | - | expression tag |
| Lys 375 | - | expression tag |
| Ile 376 | - | expression tag |
| Ser 377 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 4 | Gly 4 | variant |
| Phe 9 | Tyr 9 | conflict |
| Pro 11 | Ser 11 | conflict |
| Leu 16 | Phe 16 | conflict |
| Phe 23 | Leu 23 | conflict |
| Ile 38 | Val 38 | variant |
| Arg 44 | Gln 44 | variant |
| Leu 59 | Ala 59 | conflict |
| Ser 77 | Asn 77 | variant |
| Leu 94 | Ile 94 | conflict |
| Tyr 119 | His 119 | variant |
| Glu 175 | Lys 175 | conflict |
| Gly 194 | Val 194 | conflict |
| Leu 198 | Ile 198 | conflict |
| Ala 215 | Gly 215 | conflict |
| Arg 220 | Gln 220 | conflict |
| Val 227 | Leu 227 | variant |
| Gln 251 | Cys 251 | conflict |
| Val 256 | Ala 256 | variant |
| Ser 259 | Ile 259 | conflict |
| Leu 262 | Thr 262 | conflict |
| Leu 274 | Phe 274 | conflict |
| Pro 300 | Gln 300 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 156635.2 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 519.4 | |
| All* | Total formula weight | 157154.6 |
