8J19
Cryo-EM structure of the LY237-bound GPR84 receptor-Gi complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (R) | G-protein coupled receptor 84 | polymer | 406 | 45134.4 | 1 | UniProt (Q9NQS5) Pfam (PF00001) | Homo sapiens (human) | Inflammation-related G-protein coupled receptor EX33 |
| 2 | B (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40414.0 | 1 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 3 | C (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 348 | 38146.7 | 1 | UniProt (P62873) Pfam (PF25391) | Homo sapiens (human) | |
| 4 | D (S) | Antibody fragment ScFv16 | polymer | 269 | 28813.0 | 1 | synthetic construct | ||
| 5 | E (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 6 | F (R) | 6-nonylpyridine-2,4-diol | non-polymer | 237.3 | 1 | Chemie (SWO) |
Sequence modifications
R: 1 - 396 (UniProt: Q9NQS5)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62873)
| PDB | External Database | Details |
|---|---|---|
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Asn 47 | Ser 47 | engineered mutation |
| Ala 203 | Gly 203 | engineered mutation |
| Ala 245 | Glu 245 | engineered mutation |
| Ser 326 | Ala 326 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | expression tag |
| Ser 342 | - | expression tag |
| Ser 343 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 160369.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 237.3 | |
| All* | Total formula weight | 160606.7 |
