8J17

Crystal structure of IsPETase variant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Poly(ethylene terephthalate) hydrolase	polymer	272	28748.9	3	UniProt (A0A0K8P6T7) Pfam (PF12740)	Ideonella sakaiensis	IsPETase, PET hydrolase,PETase,PET-digesting enzyme
2	D, E, F (A, B, C)	water	water		18.0	761	Chemie (HOH)

Sequence modifications

A, B, C: -1 - 261 (UniProt: A0A0K8P6T7)

PDB	External Database	Details
Met -2	-	initiating methionine
Pro 92	Ser 121	variant
Ala 157	Asp 186	variant
Leu 262	-	expression tag
Glu 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag
His 268	-	expression tag
His 269	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	86246.8
All*	Total formula weight	86246.8

*Water molecules are not included.