8IZD
Cryo-EM structure of the C26-CoA-bound Lac1-Lip1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Ceramide synthase LAC1 | polymer | 428 | 50289.1 | 2 | UniProt (P28496) Pfam (PF03798) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (baker's yeast) | Very-long-chain ceramide synthase LAC1 |
| 2 | B, D (B, D) | Ceramide synthase subunit LIP1 | polymer | 150 | 17228.7 | 2 | UniProt (Q03579) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (baker's yeast) | LAG1/LAC1-interacting protein 1 |
| 3 | E, G, H, I, K... (A, B, C, D) | (4S,7R)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE | non-polymer | 763.1 | 6 | Chemie (6PL) | |||
| 4 | F, J (A, C) | Hexacosanoyl-CoA | non-polymer | 1146.2 | 2 | Chemie (9NY) |
Sequence modifications
A, C: 1 - 418 (UniProt: P28496)
| PDB | External Database | Details |
|---|---|---|
| Leu 419 | - | expression tag |
| Glu 420 | - | expression tag |
| Asp 421 | - | expression tag |
| Tyr 422 | - | expression tag |
| Lys 423 | - | expression tag |
| Asp 424 | - | expression tag |
| Asp 425 | - | expression tag |
| Asp 426 | - | expression tag |
| Asp 427 | - | expression tag |
| Lys 428 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 135035.5 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 6871.0 | |
| All* | Total formula weight | 141906.5 |
