8IYY

Single excitation and two emissions pH sensor protein(SITE-pHorin)_pH7.0

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green fluorescent protein	polymer	245	27677.2	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Water jellyfish Mesonema victoria)
2	B (A)	water	water		18.0	131	Chemie (HOH)

Sequence modifications

A: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	initiating methionine
Val 1	-	expression tag
Ser 48	Cys 48	engineered mutation
Leu 64	Phe 64	engineered mutation
Cro 65	Ser 65	chromophore
Cro 65	Tyr 66	chromophore
Cro 65	Gly 67	chromophore
Ala 72	Ser 72	engineered mutation
Phe 146	Asn 146	engineered mutation
Gly 148	His 148	engineered mutation
Thr 153	Met 153	engineered mutation
Ala 163	Val 163	engineered mutation
Gly 175	Ser 175	engineered mutation
Cys 203	Thr 203	engineered mutation
Lys 206	Ala 206	engineered mutation
Leu 231	His 231	engineered mutation
His 239	-	expression tag
His 240	-	expression tag
His 241	-	expression tag
His 242	-	expression tag
His 243	-	expression tag
His 244	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	27677.2
All*	Total formula weight	27677.2

*Water molecules are not included.