8IW1

Cryo-EM structure of the PEA-bound mTAAR9-Golf complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(olf) subunit alpha	polymer	256	29528.6	1	UniProt (P63096) UniProt (P38405) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein,olfactory type
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	59	6504.4	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (R)	Trace amine-associated receptor 9	polymer	348	38928.2	1	UniProt (Q5QD04) Pfam (PF00001)	Mus musculus (house mouse)	TaR-9,Trace amine receptor 9,mTaar9
5	E (S)	scFv16	polymer	285	30363.0	1		synthetic construct

Sequence modifications

A: 1 - 18 (UniProt: P63096)
A: 19 - 182 (UniProt: P38405)

PDB	External Database	Details
Asp 51	Gly 51	engineered mutation
Asn 52	Glu 52	engineered mutation

A: 191 - 381 (UniProt: P38405)

PDB	External Database	Details
Gly 183	-	linker
Gly 184	-	linker
Ser 185	-	linker
Gly 186	-	linker
Gly 187	-	linker
Ser 188	-	linker
Gly 189	-	linker
Gly 190	-	linker
Asp 236	Ala 236	engineered mutation
Asp 239	Ser 239	engineered mutation
Asp 259	Leu 259	engineered mutation
Ala 359	Ile 359	engineered mutation
Ile 362	Val 362	engineered mutation

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	initiating methionine
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	146380.2
All*	Total formula weight	146380.2

*Water molecules are not included.