8ITL

Cryo-EM structure of GIPR splice variant 1 (SV1) in complex with Gs protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	R	Gastric inhibitory polypeptide receptor	polymer	364	42252.2	1	UniProt (P48546) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (human)	GIP-R,Glucose-dependent insulinotropic polypeptide receptor
2	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45727.4	1	UniProt (P04896) Pfam (PF00503) In PDB	Bos taurus (Bovine)	Adenylate cyclase-stimulating G alpha protein
3	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	371	40227.0	1	UniProt (P54311) Pfam (PF00400) In PDB	Rattus norvegicus (Norway rat)	Transducin beta chain 1
4	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P63212) Pfam (PF00631) In PDB	Bos taurus (Bovine)	G gamma-I
5	N	Nanobody-35	polymer	128	13885.4	1		synthetic construct

Sequence modifications

R: 22 - 385 (UniProt: P48546)

PDB	External Database	Details
Phe 309	Thr 309	engineered mutation

A: 1 - 394 (UniProt: P04896)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation

B: 2 - 340 (UniProt: P54311)

PDB	External Database	Details
Met -4	-	initiating methionine
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Ser 354	-	expression tag
Gly 355	-	expression tag
Val 356	-	expression tag
Ser 357	-	expression tag
Gly 358	-	expression tag
Trp 359	-	expression tag
Arg 360	-	expression tag
Leu 361	-	expression tag
Phe 362	-	expression tag
Lys 363	-	expression tag
Lys 364	-	expression tag
Ile 365	-	expression tag
Ser 366	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	149953.3
All*	Total formula weight	149953.3

*Water molecules are not included.