8IT1

Cryo-EM structure of Crt-SPARTA-gRNA-tDNA tetramer (NADase active form)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M	Piwi domain-containing protein	polymer	507	58304.8	4	UniProt (A0A1I7NFD7) In PDB	Thermoflavifilum thermophilum	CrtAgo
2	B, F, J, N	TIR domain-containing protein	polymer	450	53256.7	4	UniProt (A0A1I7NFG5) Pfam (PF13676) In PDB	Thermoflavifilum thermophilum	CrtTIR-APAZ
3	K, O, C, G	DNA (45-mer)	polymer	45	13721.9	4		synthetic construct
4	L, P, D, H	RNA (5'-R(P*UP*GP*AP*GP*GP*UP*AP*GP*UP*AP*GP*GP*UP*UP*GP*UP*AP*UP*AP*GP*U)-3')	polymer	21	6812.0	4		synthetic construct

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	528382.0
All*	Total formula weight	528382.0

*Water molecules are not included.