8IQ6

Cryo-EM structure of Latanoprost-bound prostaglandin-F2-alpha receptor-miniGq-Nb35 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	245	28168.9	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	358	39418.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
4	D (N)	Nanobody 35	polymer	134	14686.3	1		Vicugna pacos
5	E (R)	Prostaglandin F2-alpha receptor	polymer	384	42909.4	1	UniProt (P43088) Pfam (PF00001)	Homo sapiens (human)	PGF receptor,PGF2-alpha receptor,Prostanoid FP receptor
6	F (R)	Z-7-[(1R,2R,3R,5S)-3,5-bis(oxidanyl)-2-[(3R)-3-oxidanyl-5-phenyl-pentyl]cyclopentyl]hept-5-enoic acid	non-polymer		390.5	1	Chemie (7WT)

Sequence modifications

A: 17 - 195 (UniProt: P63092)

PDB	External Database	Details
Gly 9	-	expression tag
Pro 10	-	expression tag
Thr 11	-	expression tag
Leu 12	-	expression tag
Ser 13	-	expression tag
Ala 14	-	expression tag
Glu 15	-	expression tag
Asp 16	-	expression tag
Ala 19	Gln 19	conflict
Val 20	Arg 20	conflict
Arg 22	Ala 22	conflict
Ser 23	Asn 23	conflict
Met 25	Lys 25	conflict
Asp 49	Gly 49	conflict
Asn 50	Glu 50	conflict

A: 204 - 394 (UniProt: P63092)

PDB	External Database	Details
Gly 196	-	linker
Gly 197	-	linker
Ser 198	-	linker
Gly 199	-	linker
Gly 200	-	linker
Ser 201	-	linker
Gly 202	-	linker
Gly 203	-	linker
Asp 249	Ala 249	conflict
Asp 252	Ser 252	conflict
-	Asn 254	deletion
-	Met 255	deletion
-	Val 256	deletion
-	Ile 257	deletion
-	Arg 258	deletion
-	Glu 259	deletion
-	Asp 260	deletion
-	Asn 261	deletion
-	Gln 262	deletion
-	Thr 263	deletion
Asp 272	Leu 272	conflict
Ala 372	Ile 372	conflict
Ile 375	Val 375	conflict
Lys 380	Arg 380	conflict
Leu 384	Gln 384	conflict
Gln 385	Arg 385	conflict
Asn 387	His 387	conflict
Glu 390	Gln 390	conflict
Asn 392	Glu 392	conflict
Val 394	Leu 394	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 1 - 359 (UniProt: P43088)

PDB	External Database	Details
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag
His 360	-	expression tag
His 361	-	expression tag
His 362	-	expression tag
His 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag
Gly 366	-	expression tag
Gly 367	-	expression tag
Ser 368	-	expression tag
Gly 369	-	expression tag
Gly 370	-	expression tag
Leu 371	-	expression tag
Glu 372	-	expression tag
Val 373	-	expression tag
Leu 374	-	expression tag
Phe 375	-	expression tag
Gln 376	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	133043.8
Non-Polymers*	Number of molecules	1
	Total formula weight	390.5
All*	Total formula weight	133434.4

*Water molecules are not included.