8IPR

Cryo-EM structure of heme transporter CydDC from Mycobacterium smegmatis in the outward facing ATP bound state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, D)	Component linked with the assembly of cytochrome' ABC transporter ATP-binding protein CydC	polymer	504	51880.2	2	UniProt (A0A8B4R833) Pfam (PF00664) Pfam (PF00005) UniProt (by SIFTS) (A4ZHS8)	Mycolicibacterium smegmatis
2	B, D (C, E)	Transmembrane ATP-binding protein ABC transporter cydD	polymer	515	53259.0	2	UniProt (A0A8B4R4Z5) Pfam (PF00664) Pfam (PF00005) UniProt (by SIFTS) (A0QXA6)	Mycolicibacterium smegmatis
3	E, G, I, K (A, C, D, E)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	F, H, J, L (A, C, D, E)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	4	Chemie (ATP)

Sequence modifications

A, D: 4 - 507 (UniProt: A0A8B4R833)

PDB	External Database	Details
Gln 458	Glu 456	engineered mutation

C, E: 2 - 509 (UniProt: A0A8B4R4Z5)

PDB	External Database	Details
Ser -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Val 1	-	expression tag
Gln 456	Glu 456	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	210278.5
Non-Polymers*	Number of molecules	8
	Total formula weight	2125.9
All*	Total formula weight	212404.4

*Water molecules are not included.