8IP1

Escherichia coli OpgD mutant-D388N with beta-1,2-glucan

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glucans biosynthesis protein D	polymer	559	63899.9	2	UniProt (P40120) Pfam (PF04349) In PDB	Escherichia coli (strain K12)
2	C	beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose	branched		1801.6	1	In PDB
3	E	beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose-(1-2)-beta-D-glucopyranose	branched		2125.8	1	In PDB
4	A	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
5	B	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
6		water	water		18.0	566	Chemie (HOH)

Sequence modifications

A, B: 1 - 551 (UniProt: P40120)

PDB	External Database	Details
Asn 388	Asp 388	engineered mutation
Leu 552	-	expression tag
Glu 553	-	expression tag
His 554	-	expression tag
His 555	-	expression tag
His 556	-	expression tag
His 557	-	expression tag
His 558	-	expression tag
His 559	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	127799.9
Branched	Number of molecules	2
	Total formula weight	3927.4
Non-Polymers*	Number of molecules	3
	Total formula weight	362.4
All*	Total formula weight	132089.7

*Water molecules are not included.