8IOC
Cryo-EM structure of the gamma-MSH-bound human melanocortin receptor 3 (MC3R)-Gs complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 361 | 41879.5 | 1 | UniProt (P63096) UniProt (P63092) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,HiBiT | polymer | 371 | 40227.0 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 3 | C (R) | HA signal peptide,Melanocortin receptor 3,LgBiT subunit | polymer | 511 | 56351.9 | 1 | UniProt (P03435) UniProt (P41968) Pfam (PF00001) | Influenza A virus (strain A/Victoria/3/1975 H3N2) | MC3-R |
| 4 | D (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (cattle) | G gamma-I |
| 5 | E (L) | gamma-melanocyte-stimulating hormone | polymer | 11 | 1516.7 | 1 | Pfam (PF00976) UniProt (by SIFTS) (P01189) | synthetic construct | |
| 6 | F (N) | Nanobody-35 | polymer | 160 | 17352.5 | 1 | Homo sapiens (human) | ||
| 7 | G (R) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 8 - 25 (UniProt: P63096)
A: 26 - 82 (UniProt: P63092)
A: 83 - 203 (UniProt: P63096)
A: 204 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
B: 356 - 366 (PDB: 8IOC)
R: -14 - 1 (UniProt: P03435)
R: 2 - 323 (UniProt: P41968)
R: 339 - 496 (PDB: 8IOC)
A: 26 - 82 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Asp 49 | Gly 49 | engineered mutation |
| Asn 50 | Glu 50 | engineered mutation |
| Tyr 63 | Leu 63 | engineered mutation |
A: 204 - 394 (UniProt: P63092)
| PDB | External Database | Details |
|---|---|---|
| Ala 226 | Gly 226 | engineered mutation |
| Asp 249 | Ala 249 | engineered mutation |
| Asp 252 | Ser 252 | engineered mutation |
| - | Asn 254 | deletion |
| - | Met 255 | deletion |
| - | Val 256 | deletion |
| - | Ile 257 | deletion |
| - | Arg 258 | deletion |
| - | Glu 259 | deletion |
| - | Asp 260 | deletion |
| - | Asn 261 | deletion |
| - | Gln 262 | deletion |
| - | Thr 263 | deletion |
| Asp 272 | Leu 272 | engineered mutation |
| Ser 366 | Ala 366 | engineered mutation |
| Ala 372 | Ile 372 | engineered mutation |
| Ile 375 | Val 375 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -4 | - | initiating methionine |
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| Leu -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Gly 341 | - | linker |
| Ser 342 | - | linker |
| Ser 343 | - | linker |
| Gly 344 | - | linker |
| Gly 345 | - | linker |
| Gly 346 | - | linker |
| Gly 347 | - | linker |
| Ser 348 | - | linker |
| Gly 349 | - | linker |
| Gly 350 | - | linker |
| Gly 351 | - | linker |
| Gly 352 | - | linker |
| Ser 353 | - | linker |
| Ser 354 | - | linker |
| Gly 355 | - | linker |
R: -14 - 1 (UniProt: P03435)
R: 2 - 323 (UniProt: P41968)
| PDB | External Database | Details |
|---|---|---|
| Gly 324 | - | linker |
| Ser 325 | - | linker |
| Ser 326 | - | linker |
| Gly 327 | - | linker |
| Gly 328 | - | linker |
| Gly 329 | - | linker |
| Gly 330 | - | linker |
| Ser 331 | - | linker |
| Gly 332 | - | linker |
| Gly 333 | - | linker |
| Gly 334 | - | linker |
| Gly 335 | - | linker |
| Ser 336 | - | linker |
| Ser 337 | - | linker |
| Gly 338 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 165188.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 40.1 | |
| All* | Total formula weight | 165228.8 |
