8IOC
Cryo-EM structure of the gamma-MSH-bound human melanocortin receptor 3 (MC3R)-Gs complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1,Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 361 | 41879.5 | 1 | UniProt (P63096) UniProt (P63092) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein,Adenylate cyclase-stimulating G alpha protein |
2 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1,HiBiT | polymer | 371 | 40227.0 | 1 | UniProt (P62873) Pfam (PF00400) In PDB | Homo sapiens (human) | Transducin beta chain 1 |
3 | R | HA signal peptide,Melanocortin receptor 3,LgBiT subunit | polymer | 511 | 56351.9 | 1 | UniProt (P03435) UniProt (P41968) Pfam (PF00001) In PDB | Influenza A virus (strain A/Victoria/3/1975 H3N2) | MC3-R |
4 | G | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7861.1 | 1 | UniProt (P63212) Pfam (PF00631) In PDB | Bos taurus (cattle) | G gamma-I |
5 | L | gamma-melanocyte-stimulating hormone | polymer | 11 | 1516.7 | 1 | Pfam (PF00976) UniProt (by SIFTS) (P01189) In PDB | synthetic construct | |
6 | N | Nanobody-35 | polymer | 160 | 17352.5 | 1 | Homo sapiens (human) | ||
7 | R | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 8 - 25 (UniProt: P63096)
A: 26 - 82 (UniProt: P63092)
A: 83 - 203 (UniProt: P63096)
A: 204 - 394 (UniProt: P63092)
B: 2 - 340 (UniProt: P62873)
B: 356 - 366 (PDB: 8IOC)
R: -14 - 1 (UniProt: P03435)
R: 2 - 323 (UniProt: P41968)
R: 339 - 496 (PDB: 8IOC)
A: 26 - 82 (UniProt: P63092)
PDB | External Database | Details |
---|---|---|
Asp 49 | Gly 49 | engineered mutation |
Asn 50 | Glu 50 | engineered mutation |
Tyr 63 | Leu 63 | engineered mutation |
A: 204 - 394 (UniProt: P63092)
PDB | External Database | Details |
---|---|---|
Ala 226 | Gly 226 | engineered mutation |
Asp 249 | Ala 249 | engineered mutation |
Asp 252 | Ser 252 | engineered mutation |
- | Asn 254 | deletion |
- | Met 255 | deletion |
- | Val 256 | deletion |
- | Ile 257 | deletion |
- | Arg 258 | deletion |
- | Glu 259 | deletion |
- | Asp 260 | deletion |
- | Asn 261 | deletion |
- | Gln 262 | deletion |
- | Thr 263 | deletion |
Asp 272 | Leu 272 | engineered mutation |
Ser 366 | Ala 366 | engineered mutation |
Ala 372 | Ile 372 | engineered mutation |
Ile 375 | Val 375 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -4 | - | initiating methionine |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Gly 341 | - | linker |
Ser 342 | - | linker |
Ser 343 | - | linker |
Gly 344 | - | linker |
Gly 345 | - | linker |
Gly 346 | - | linker |
Gly 347 | - | linker |
Ser 348 | - | linker |
Gly 349 | - | linker |
Gly 350 | - | linker |
Gly 351 | - | linker |
Gly 352 | - | linker |
Ser 353 | - | linker |
Ser 354 | - | linker |
Gly 355 | - | linker |
R: -14 - 1 (UniProt: P03435)
R: 2 - 323 (UniProt: P41968)
PDB | External Database | Details |
---|---|---|
Gly 324 | - | linker |
Ser 325 | - | linker |
Ser 326 | - | linker |
Gly 327 | - | linker |
Gly 328 | - | linker |
Gly 329 | - | linker |
Gly 330 | - | linker |
Ser 331 | - | linker |
Gly 332 | - | linker |
Gly 333 | - | linker |
Gly 334 | - | linker |
Gly 335 | - | linker |
Ser 336 | - | linker |
Ser 337 | - | linker |
Gly 338 | - | linker |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 165188.8 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 40.1 | |
All* | Total formula weight | 165228.8 |