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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

8IMX

Cryo-EM structure of GPI-T with a chimeric GPI-anchored protein

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(D)		UL16-binding protein 2,GFP-like fluorescent chromoprotein cFP484polymer32735573.31UniProt (Q9BZM5)
UniProt (Q9U6Y3)
Pfam (PF00129)		Homo sapiens (human)ALCAN-alpha,NKG2D ligand 2,N2DL-2,NKG2DL2,Retinoic acid early transcript 1H
2B
(G)		Glycosylphosphatidylinositol anchor attachment 1 protein,GFP-like fluorescent chromoprotein cFP484polymer88696990.41UniProt (O43292)
UniProt (Q9U6Y3)
Pfam (PF04114)		Homo sapiens (human)GPI anchor attachment protein 1,GAA1 protein homolog,hGAA1
3C
(K)		GPI-anchor transamidase,GFP-like fluorescent chromoprotein cFP484polymer64773443.11UniProt (Q92643)
UniProt (Q9U6Y3)
Pfam (PF01650)		Homo sapiens (human)GPI transamidase,GPI8 homolog,hGPI8,Phosphatidylinositol-glycan biosynthesis class K protein,PIG-K
4D
(S)		GPI transamidase component PIG-S,GFP-like fluorescent chromoprotein cFP484polymer81690421.11UniProt (Q96S52)
UniProt (Q9U6Y3)
Pfam (PF10510)		Homo sapiens (human)Phosphatidylinositol-glycan biosynthesis class S protein
5E
(T)		GPI transamidase component PIG-T,GFP-like fluorescent chromoprotein cFP484polymer83194207.31UniProt (Q969N2)
UniProt (Q9U6Y3)
Pfam (PF04113)
Pfam (PF01353)		Homo sapiens (human)Phosphatidylinositol-glycan biosynthesis class T protein
6F
(U)		Phosphatidylinositol glycan anchor biosynthesis class U protein,GFP-like fluorescent chromoprotein cFP484polymer67877141.71UniProt (Q9H490)
UniProt (Q9U6Y3)
Pfam (PF06728)		Homo sapiens (human)Cell division cycle protein 91-like 1,Protein CDC91-like 1,GPI transamidase component PIG-U
7G
(P)		Met-Ser-Ser peptidepolymer3323.41synthetic construct
8H
(R)		2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranosebranched424.41In PDB
GlyTouCan (G42666HT)
9BA, CA, DA, EA, FA...
(U, D, G)		CHOLESTEROL HEMISUCCINATEnon-polymer486.713Chemie (Y01)
10J
(G)		MAGNESIUM IONnon-polymer24.31Chemie (MG)
11L, M, N, Z
(G, U)		[(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoatenon-polymer718.04Chemie (6OU)
12U
(G)		Digitoninnon-polymer1229.31Chemie (AJP)
13V
(K)		CALCIUM IONnon-polymer40.11Chemie (CA)
14W, Y
(S, T)		2-acetamido-2-deoxy-beta-D-glucopyranosenon-polymer221.22Chemie (NAG)
15X
(S)		1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholinenon-polymer760.11Chemie (LBN)
16AA
(U)		[(2R)-1-hexadecanoyloxy-3-[[3-[[(2R)-3-hexadecanoyloxy-2-[(Z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (Z)-octadec-9-enoatenon-polymer1405.91Chemie (80T)
17GA, IA
(P)		2-azanylethyl [(2R,3S,4S,5S,6S)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphatenon-polymer303.22Chemie (80Y)
18HA
(P)		2-amino-2-deoxy-alpha-D-glucopyranosenon-polymer179.21Chemie (PA1)
19JA
(P)		2-azanylethyl [(2~{S},3~{S},4~{S},5~{S},6~{R})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphatenon-polymer303.21Chemie (05E)
20KA
(P)		[(2R)-1-[[(1S,2R,3R,4S,5S,6R)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5Z,8Z,11Z,14Z)-icosa-5,8,11,14-tetraenoatenon-polymer1111.61Chemie (81Q)
Sequence modifications
D: -79 - -56 (UniProt: Q9BZM5)
PDBExternal DatabaseDetails
Met -80-initiating methionine
D: -47 - 168 (UniProt: Q9U6Y3)
PDBExternal DatabaseDetails
Glu -43Asp 49conflict
Arg -37Lys 55conflict
Ala -33Asn 59conflict
Lys -28Ala 64conflict
Ile -20Glu 72conflict
Glu -15Asp 77conflict
Gln -12His 80conflict
Asp -9Asn 83conflict
Thr -7Glu 85conflict
Glu -5Lys 87conflict
Thr 8Ser 100conflict
Pro 9Asn 101conflict
Phe 18Leu 110conflict
Glu 23Asp 115conflict
Pro 26Ala 118conflict
Ala 32Ser 124conflict
Ser 42Thr 134conflict
Tyr 45Phe 137conflict
Gln 48Lys 140conflict
Cys 51Val 143conflict
Ile 52Lys 144conflict
Ala 53Val 145conflict
Thr 54Lys 146conflict
Thr 58Ser 150conflict
Gly 61Glu 153conflict
Cys 63Ser 155conflict
Phe 65Ile 157conflict
Thr 73Met 165conflict
Lys 95Ile 187conflict
Glu 99Arg 191conflict
Lys 104Val 196conflict
Val 107Ile 199conflict
Glu 108Ser 200conflict
Met 109His 201conflict
Ala 110Ser 202conflict
Thr 125Ser 217conflict
Thr 126Ile 218conflict
Asp 132Val 224conflict
Arg 134Lys 226conflict
Ala 138Tyr 230conflict
Glu 140Phe 232conflict
Ser 149Asn 241conflict
Arg 158Thr 250conflict
His 162Asn 254conflict
Glu 164Val 256conflict
Gly 169-linker
Gly 170-linker
Gly 171-linker
Ser 172-linker
Gly 173-linker
Gly 174-linker
Gly 175-linker
Ser 176-linker
Ala 177-linker
Trp 178-linker
Ser 179-linker
His 180-linker
Pro 181-linker
Gln 182-linker
Phe 183-linker
Glu 184-linker
Lys 185-linker
Gly 186-linker
Gly 187-linker
Gly 188-linker
Ser 189-linker
Gly 190-linker
Gly 191-linker
Gly 192-linker
Ser 193-linker
Gly 194-linker
Gly 195-linker
Ser 196-linker
Ala 197-linker
Trp 198-linker
Ser 199-linker
His 200-linker
Pro 201-linker
Gln 202-linker
Phe 203-linker
Glu 204-linker
Lys 205-linker
Gly 206-linker
Ser 207-linker
D: 208 - 246 (UniProt: Q9BZM5)
PDBExternal DatabaseDetails
Gly -55-linker
Gly -54-linker
Ser -53-linker
Gly -52-linker
Gly -51-linker
Ser -50-linker
Ala -49-linker
Ser -48-linker
G: 2 - 621 (UniProt: O43292)
PDBExternal DatabaseDetails
Met -1-initiating methionine
Gly 0-expression tag
Ser 1-expression tag
Gly 622-linker
Thr 623-linker
Leu 624-linker
Glu 625-linker
Val 626-linker
Leu 627-linker
Phe 628-linker
Gln 629-linker
Gly 630-linker
Pro 631-linker
Gly 632-linker
Gly 633-linker
Ser 634-linker
Gly 635-linker
Gly 636-linker
Ser 637-linker
Ala 638-linker
Ser 639-linker
G: 640 - 855 (UniProt: Q9U6Y3)
PDBExternal DatabaseDetails
Glu 644Asp 49conflict
Arg 650Lys 55conflict
Ala 654Asn 59conflict
Lys 659Ala 64conflict
Ile 667Glu 72conflict
Glu 672Asp 77conflict
Gln 675His 80conflict
Asp 678Asn 83conflict
Thr 680Glu 85conflict
Glu 682Lys 87conflict
Thr 695Ser 100conflict
Pro 696Asn 101conflict
Phe 705Leu 110conflict
Glu 710Asp 115conflict
Pro 713Ala 118conflict
Ala 719Ser 124conflict
Ser 729Thr 134conflict
Tyr 732Phe 137conflict
Gln 735Lys 140conflict
Cys 738Val 143conflict
Ile 739Lys 144conflict
Ala 740Val 145conflict
Thr 741Lys 146conflict
Thr 745Ser 150conflict
Gly 748Glu 153conflict
Cys 750Ser 155conflict
Phe 752Ile 157conflict
Thr 760Met 165conflict
Lys 782Ile 187conflict
Glu 786Arg 191conflict
Lys 791Val 196conflict
Val 794Ile 199conflict
Glu 795Ser 200conflict
Met 796His 201conflict
Ala 797Ser 202conflict
Thr 812Ser 217conflict
Thr 813Ile 218conflict
Asp 819Val 224conflict
Arg 821Lys 226conflict
Ala 825Tyr 230conflict
Glu 827Phe 232conflict
Ser 836Asn 241conflict
Arg 845Thr 250conflict
His 849Asn 254conflict
Glu 851Val 256conflict
Gly 856-expression tag
Gly 857-expression tag
Gly 858-expression tag
Ser 859-expression tag
Gly 860-expression tag
Gly 861-expression tag
Gly 862-expression tag
Gly 863-expression tag
Ser 864-expression tag
Gly 865-expression tag
Gly 866-expression tag
Gly 867-expression tag
Gly 868-expression tag
Asp 869-expression tag
Tyr 870-expression tag
Lys 871-expression tag
Asp 872-expression tag
Asp 873-expression tag
Asp 874-expression tag
Asp 875-expression tag
Ala 876-expression tag
Asp 877-expression tag
Tyr 878-expression tag
Lys 879-expression tag
Asp 880-expression tag
Asp 881-expression tag
Asp 882-expression tag
Asp 883-expression tag
Ala 884-expression tag
K: 2 - 395 (UniProt: Q92643)
PDBExternal DatabaseDetails
Met -1-initiating methionine
Gly 0-expression tag
Ser 1-expression tag
Gly 396-linker
Thr 397-linker
Leu 398-linker
Glu 399-linker
Val 400-linker
Leu 401-linker
Phe 402-linker
Gln 403-linker
Gly 404-linker
Pro 405-linker
Gly 406-linker
Gly 407-linker
Ser 408-linker
Gly 409-linker
Gly 410-linker
Ser 411-linker
Ala 412-linker
Ser 413-linker
K: 414 - 629 (UniProt: Q9U6Y3)
PDBExternal DatabaseDetails
Glu 418Asp 49conflict
Arg 424Lys 55conflict
Ala 428Asn 59conflict
Lys 433Ala 64conflict
Ile 441Glu 72conflict
Glu 446Asp 77conflict
Gln 449His 80conflict
Asp 452Asn 83conflict
Thr 454Glu 85conflict
Glu 456Lys 87conflict
Thr 469Ser 100conflict
Pro 470Asn 101conflict
Phe 479Leu 110conflict
Glu 484Asp 115conflict
Pro 487Ala 118conflict
Ala 493Ser 124conflict
Ser 503Thr 134conflict
Tyr 506Phe 137conflict
Gln 509Lys 140conflict
Cys 512Val 143conflict
Ile 513Lys 144conflict
Ala 514Val 145conflict
Thr 515Lys 146conflict
Thr 519Ser 150conflict
Gly 522Glu 153conflict
Cys 524Ser 155conflict
Phe 526Ile 157conflict
Thr 534Met 165conflict
Lys 556Ile 187conflict
Glu 560Arg 191conflict
Lys 565Val 196conflict
Val 568Ile 199conflict
Glu 569Ser 200conflict
Met 570His 201conflict
Ala 571Ser 202conflict
Thr 586Ser 217conflict
Thr 587Ile 218conflict
Asp 593Val 224conflict
Arg 595Lys 226conflict
Ala 599Tyr 230conflict
Glu 601Phe 232conflict
Ser 610Asn 241conflict
Arg 619Thr 250conflict
His 623Asn 254conflict
Glu 625Val 256conflict
Gly 630-expression tag
Gly 631-expression tag
Gly 632-expression tag
Ser 633-expression tag
Gly 634-expression tag
Gly 635-expression tag
Gly 636-expression tag
Tyr 637-expression tag
Pro 638-expression tag
Tyr 639-expression tag
Asp 640-expression tag
Val 641-expression tag
Pro 642-expression tag
Asp 643-expression tag
Tyr 644-expression tag
Ala 645-expression tag
S: 2 - 555 (UniProt: Q96S52)
PDBExternal DatabaseDetails
Met -1-initiating methionine
Gly 0-expression tag
Ser 1-expression tag
Gly 556-linker
Thr 557-linker
Leu 558-linker
Glu 559-linker
Val 560-linker
Leu 561-linker
Phe 562-linker
Gln 563-linker
Gly 564-linker
Pro 565-linker
Gly 566-linker
Gly 567-linker
Ser 568-linker
Gly 569-linker
Gly 570-linker
Ser 571-linker
Ala 572-linker
Ser 573-linker
S: 574 - 789 (UniProt: Q9U6Y3)
PDBExternal DatabaseDetails
Glu 578Asp 49conflict
Arg 584Lys 55conflict
Ala 588Asn 59conflict
Lys 593Ala 64conflict
Ile 601Glu 72conflict
Glu 606Asp 77conflict
Gln 609His 80conflict
Asp 612Asn 83conflict
Thr 614Glu 85conflict
Glu 616Lys 87conflict
Thr 629Ser 100conflict
Pro 630Asn 101conflict
Phe 639Leu 110conflict
Glu 644Asp 115conflict
Pro 647Ala 118conflict
Ala 653Ser 124conflict
Ser 663Thr 134conflict
Tyr 666Phe 137conflict
Gln 669Lys 140conflict
Cys 672Val 143conflict
Ile 673Lys 144conflict
Ala 674Val 145conflict
Thr 675Lys 146conflict
Thr 679Ser 150conflict
Gly 682Glu 153conflict
Cys 684Ser 155conflict
Phe 686Ile 157conflict
Thr 694Met 165conflict
Lys 716Ile 187conflict
Glu 720Arg 191conflict
Lys 725Val 196conflict
Val 728Ile 199conflict
Glu 729Ser 200conflict
Met 730His 201conflict
Ala 731Ser 202conflict
Thr 746Ser 217conflict
Thr 747Ile 218conflict
Asp 753Val 224conflict
Arg 755Lys 226conflict
Ala 759Tyr 230conflict
Glu 761Phe 232conflict
Ser 770Asn 241conflict
Arg 779Thr 250conflict
His 783Asn 254conflict
Glu 785Val 256conflict
Gly 790-expression tag
Gly 791-expression tag
Gly 792-expression tag
Ser 793-expression tag
Gly 794-expression tag
Gly 795-expression tag
Gly 796-expression tag
Gly 797-expression tag
Gly 798-expression tag
Gly 799-expression tag
Gly 800-expression tag
Gly 801-expression tag
Gly 802-expression tag
Gly 803-expression tag
Gly 804-expression tag
Glu 805-expression tag
Gln 806-expression tag
Lys 807-expression tag
Leu 808-expression tag
Ile 809-expression tag
Ser 810-expression tag
Glu 811-expression tag
Glu 812-expression tag
Asp 813-expression tag
Leu 814-expression tag
T: 2 - 578 (UniProt: Q969N2)
PDBExternal DatabaseDetails
Met -1-initiating methionine
Gly 0-expression tag
Ser 1-expression tag
Gly 579-linker
Thr 580-linker
Leu 581-linker
Glu 582-linker
Val 583-linker
Leu 584-linker
Phe 585-linker
Gln 586-linker
Gly 587-linker
Pro 588-linker
Gly 589-linker
Gly 590-linker
Ser 591-linker
Gly 592-linker
Gly 593-linker
Ser 594-linker
Ala 595-linker
Ser 596-linker
T: 597 - 812 (UniProt: Q9U6Y3)
PDBExternal DatabaseDetails
Glu 601Asp 49conflict
Arg 607Lys 55conflict
Ala 611Asn 59conflict
Lys 616Ala 64conflict
Ile 624Glu 72conflict
Glu 629Asp 77conflict
Gln 632His 80conflict
Asp 635Asn 83conflict
Thr 637Glu 85conflict
Glu 639Lys 87conflict
Thr 652Ser 100conflict
Pro 653Asn 101conflict
Phe 662Leu 110conflict
Glu 667Asp 115conflict
Pro 670Ala 118conflict
Ala 676Ser 124conflict
Ser 686Thr 134conflict
Tyr 689Phe 137conflict
Gln 692Lys 140conflict
Cys 695Val 143conflict
Ile 696Lys 144conflict
Ala 697Val 145conflict
Thr 698Lys 146conflict
Thr 702Ser 150conflict
Gly 705Glu 153conflict
Cys 707Ser 155conflict
Phe 709Ile 157conflict
Thr 717Met 165conflict
Lys 739Ile 187conflict
Glu 743Arg 191conflict
Lys 748Val 196conflict
Val 751Ile 199conflict
Glu 752Ser 200conflict
Met 753His 201conflict
Ala 754Ser 202conflict
Thr 769Ser 217conflict
Thr 770Ile 218conflict
Asp 776Val 224conflict
Arg 778Lys 226conflict
Ala 782Tyr 230conflict
Glu 784Phe 232conflict
Ser 793Asn 241conflict
Arg 802Thr 250conflict
His 806Asn 254conflict
Glu 808Val 256conflict
Gly 813-expression tag
Gly 814-expression tag
Gly 815-expression tag
Ser 816-expression tag
Gly 817-expression tag
Gly 818-expression tag
Gly 819-expression tag
His 820-expression tag
His 821-expression tag
His 822-expression tag
His 823-expression tag
His 824-expression tag
His 825-expression tag
His 826-expression tag
His 827-expression tag
His 828-expression tag
His 829-expression tag
U: 2 - 435 (UniProt: Q9H490)
PDBExternal DatabaseDetails
Met -1-initiating methionine
Gly 0-expression tag
Ser 1-expression tag
Gly 436-linker
Thr 437-linker
Leu 438-linker
Glu 439-linker
Val 440-linker
Leu 441-linker
Phe 442-linker
Gln 443-linker
Gly 444-linker
Pro 445-linker
Gly 446-linker
Gly 447-linker
Ser 448-linker
Gly 449-linker
Gly 450-linker
Ser 451-linker
Ala 452-linker
Ser 453-linker
U: 454 - 669 (UniProt: Q9U6Y3)
PDBExternal DatabaseDetails
Glu 458Asp 49conflict
Arg 464Lys 55conflict
Ala 468Asn 59conflict
Lys 473Ala 64conflict
Ile 481Glu 72conflict
Glu 486Asp 77conflict
Gln 489His 80conflict
Asp 492Asn 83conflict
Thr 494Glu 85conflict
Glu 496Lys 87conflict
Thr 509Ser 100conflict
Pro 510Asn 101conflict
Phe 519Leu 110conflict
Glu 524Asp 115conflict
Pro 527Ala 118conflict
Ala 533Ser 124conflict
Ser 543Thr 134conflict
Tyr 546Phe 137conflict
Gln 549Lys 140conflict
Cys 552Val 143conflict
Ile 553Lys 144conflict
Ala 554Val 145conflict
Thr 555Lys 146conflict
Thr 559Ser 150conflict
Gly 562Glu 153conflict
Cys 564Ser 155conflict
Phe 566Ile 157conflict
Thr 574Met 165conflict
Lys 596Ile 187conflict
Glu 600Arg 191conflict
Lys 605Val 196conflict
Val 608Ile 199conflict
Glu 609Ser 200conflict
Met 610His 201conflict
Ala 611Ser 202conflict
Thr 626Ser 217conflict
Thr 627Ile 218conflict
Asp 633Val 224conflict
Arg 635Lys 226conflict
Ala 639Tyr 230conflict
Glu 641Phe 232conflict
Ser 650Asn 241conflict
Arg 659Thr 250conflict
His 663Asn 254conflict
Glu 665Val 256conflict
Gly 670-expression tag
Gly 671-expression tag
Gly 672-expression tag
Ser 673-expression tag
Gly 674-expression tag
Gly 675-expression tag
Gly 676-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains7
Total formula weight468100.1
BranchedNumber of molecules1
Total formula weight424.4
Non-Polymers*Number of molecules29
Total formula weight15301.9
All*Total formula weight483826.4
*Water molecules are not included.

243911

PDB entries from 2025-10-29

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