8IJO

Structure of DNA binding domain of McrBC endonuclease bound to DNA: Y41F-L68F double mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Type IV methyl-directed restriction enzyme EcoKMcrB subunit	polymer	170	19677.9	2	UniProt (P15005) Pfam (PF12102)	Escherichia coli K-12	EcoKMcrBC,5-methylcytosine-specific restriction enzyme B
2	C (C)	DNA (5'-D(*GP*AP*GP*AP*CP*CP*GP*GP*TP*AP*GP*C)-3')	polymer	13	4016.6	1		DNA molecule
3	D (D)	DNA (5'-D(*GP*CP*TP*AP*CP*CP*GP*GP*TP*CP*TP*C)-3')	polymer	13	3927.6	1		DNA molecule
4	E, F (C, D)	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	2	Chemie (BTB)
5	G, H, I, J (A, B, C, D)	water	water		18.0	325	Chemie (HOH)

Sequence modifications

A, B: 1 - 161 (UniProt: P15005)

PDB	External Database	Details
Phe 41	Tyr 41	engineered mutation
Phe 68	Leu 68	engineered mutation
Leu 162	-	expression tag
Glu 163	-	expression tag
Gly 164	-	expression tag
His 165	-	expression tag
His 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	47300.1
Non-Polymers*	Number of molecules	2
	Total formula weight	418.5
All*	Total formula weight	47718.5

*Water molecules are not included.