8IJM

Cyo-EM structure of K794A non-gastric proton pump in Na+ bound E1AMPPCP state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Sodium/potassium-transporting ATPase subunit alpha	polymer	986	109084.6	1	UniProt (G3V8S4) Pfam (PF00690) Pfam (PF00122) Pfam (PF13246) Pfam (PF00702) Pfam (PF00689) UniProt (by SIFTS) (P54708) In PDB	Rattus
2	B	Sodium/potassium-transporting ATPase subunit beta-1	polymer	324	37516.9	1	UniProt (P07340) Pfam (PF00287) In PDB	Rattus	Sodium/potassium-dependent ATPase subunit beta-1
3	A	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	1	Chemie (ACP)
4	A	1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE	non-polymer		787.1	2	Chemie (PCW)
5	A	CHOLESTEROL	non-polymer		386.7	1	Chemie (CLR)
6	A	SODIUM ION	non-polymer		23.0	2	Chemie (NA)
7	B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A: 53 - 1036 (UniProt: G3V8S4)

PDB	External Database	Details
Gly 51	-	expression tag
Met 52	-	expression tag
Asp 315	Gly 315	engineered mutation
Asn 512	Asp 512	engineered mutation
Ala 794	Lys 794	engineered mutation

B: 1 - 304 (UniProt: P07340)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Asp -17	-	expression tag
Tyr -16	-	expression tag
Lys -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Lys -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Glu -6	-	expression tag
Asn -5	-	expression tag
Leu -4	-	expression tag
Tyr -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	146601.5
Non-Polymers*	Number of molecules	7
	Total formula weight	2733.3
All*	Total formula weight	149334.8

*Water molecules are not included.