8IJL
Cyo-EM structure of wildtype non-gastric proton pump in the presence of Na+, AlF and ADP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Sodium/potassium-transporting ATPase subunit alpha | polymer | 986 | 109084.7 | 1 | UniProt (G3V8S4) Pfam (PF00690) Pfam (PF00122) Pfam (PF13246) Pfam (PF00702) Pfam (PF00689) UniProt (by SIFTS) (P54708) In PDB | Rattus | |
2 | B | Sodium/potassium-transporting ATPase subunit beta-1 | polymer | 324 | 37516.9 | 1 | UniProt (P07340) Pfam (PF00287) In PDB | Rattus | Sodium/potassium-dependent ATPase subunit beta-1 |
3 | A | TETRAFLUOROALUMINATE ION | non-polymer | 103.0 | 1 | Chemie (ALF) | |||
4 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
5 | A | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
6 | A | 1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 787.1 | 5 | Chemie (PCW) | |||
7 | A, B | CHOLESTEROL | non-polymer | 386.7 | 2 | Chemie (CLR) | |||
8 | A | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
9 | A, B | 2-{[(4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25R)-spirost-5-en-3-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside | non-polymer | 1165.3 | 4 | Chemie (Q7G) | |||
10 | B | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
11 | water | water | 18.0 | 8 | Chemie (HOH) |
Sequence modifications
A: 53 - 1036 (UniProt: G3V8S4)
B: 1 - 304 (UniProt: P07340)
PDB | External Database | Details |
---|---|---|
Gly 51 | - | expression tag |
Met 52 | - | expression tag |
Asn 512 | Asp 512 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Asp -17 | - | expression tag |
Tyr -16 | - | expression tag |
Lys -15 | - | expression tag |
Asp -14 | - | expression tag |
Asp -13 | - | expression tag |
Asp -12 | - | expression tag |
Asp -11 | - | expression tag |
Lys -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Glu -6 | - | expression tag |
Asn -5 | - | expression tag |
Leu -4 | - | expression tag |
Tyr -3 | - | expression tag |
Phe -2 | - | expression tag |
Gln -1 | - | expression tag |
Gly 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 146601.5 | |
Non-Polymers* | Number of molecules | 17 |
Total formula weight | 10191.8 | |
All* | Total formula weight | 156793.4 |