8IHJ
Cryo-EM structure of HCA3-Gi complex with acifran
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | R | Soluble cytochrome b562,Hydroxycarboxylic acid receptor 3 | polymer | 530 | 60749.4 | 1 | UniProt (P0ABE7) UniProt (P49019) Pfam (PF07361) Pfam (PF00001) In PDB | Escherichia coli | Cytochrome b-562,G-protein coupled receptor 109B,G-protein coupled receptor HM74,G-protein coupled receptor HM74B,Niacin receptor 2,Nicotinic acid receptor 2 |
2 | A | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 354 | 40446.0 | 1 | UniProt (P63096) Pfam (PF00503) In PDB | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
3 | S | scFv16 | polymer | 248 | 26496.5 | 1 | synthetic construct | ||
4 | B | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 382 | 41772.6 | 1 | UniProt (P62874) Pfam (PF00400) In PDB | Mus musculus (house mouse) | |
5 | C | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7729.9 | 1 | UniProt (P63213) Pfam (PF00631) In PDB | Mus musculus (house mouse) | G gamma-I |
6 | R | (5~{S})-5-methyl-4-oxidanylidene-5-phenyl-furan-2-carboxylic acid | non-polymer | 218.2 | 1 | Chemie (P9X) |
Sequence modifications
R: -104 - 0 (UniProt: P0ABE7)
R: 2 - 387 (UniProt: P49019)
A: 1 - 354 (UniProt: P63096)
B: 2 - 340 (UniProt: P62874)
PDB | External Database | Details |
---|---|---|
Met -128 | - | initiating methionine |
Lys -127 | - | expression tag |
Thr -126 | - | expression tag |
Ile -125 | - | expression tag |
Ile -124 | - | expression tag |
Ala -123 | - | expression tag |
Leu -122 | - | expression tag |
Ser -121 | - | expression tag |
Tyr -120 | - | expression tag |
Ile -119 | - | expression tag |
Phe -118 | - | expression tag |
Cys -117 | - | expression tag |
Leu -116 | - | expression tag |
Val -115 | - | expression tag |
Phe -114 | - | expression tag |
Ala -113 | - | expression tag |
Asp -112 | - | expression tag |
Tyr -111 | - | expression tag |
Lys -110 | - | expression tag |
Asp -109 | - | expression tag |
Asp -108 | - | expression tag |
Asp -107 | - | expression tag |
Asp -106 | - | expression tag |
Lys -105 | - | expression tag |
Trp -98 | Met 29 | engineered mutation |
Ile -3 | His 124 | engineered mutation |
Leu 1 | - | linker |
PDB | External Database | Details |
---|---|---|
Glu 388 | - | expression tag |
Asn 389 | - | expression tag |
Leu 390 | - | expression tag |
Tyr 391 | - | expression tag |
Phe 392 | - | expression tag |
Gln 393 | - | expression tag |
Gly 394 | - | expression tag |
Ser 395 | - | expression tag |
His 396 | - | expression tag |
His 397 | - | expression tag |
His 398 | - | expression tag |
His 399 | - | expression tag |
His 400 | - | expression tag |
His 401 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Ala 203 | Gly 203 | engineered mutation |
Ala 219 | Thr 219 | conflict |
Gln 288 | Pro 288 | conflict |
Ser 326 | Ala 326 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Met -15 | - | initiating methionine |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
Glu -6 | - | expression tag |
Asn -5 | - | expression tag |
Leu -4 | - | expression tag |
Tyr -3 | - | expression tag |
Phe -2 | - | expression tag |
Gln -1 | - | expression tag |
Gly 0 | - | expression tag |
Ser 1 | - | expression tag |
Gly 341 | - | expression tag |
Gly 342 | - | expression tag |
Ser 343 | - | expression tag |
Gly 344 | - | expression tag |
Gly 345 | - | expression tag |
Gly 346 | - | expression tag |
Gly 347 | - | expression tag |
Ser 348 | - | expression tag |
Gly 349 | - | expression tag |
Gly 350 | - | expression tag |
Ser 351 | - | expression tag |
Ser 352 | - | expression tag |
Ser 353 | - | expression tag |
Gly 354 | - | expression tag |
Gly 355 | - | expression tag |
Val 356 | - | expression tag |
Ser 357 | - | expression tag |
Gly 358 | - | expression tag |
Trp 359 | - | expression tag |
Arg 360 | - | expression tag |
Leu 361 | - | expression tag |
Phe 362 | - | expression tag |
Lys 363 | - | expression tag |
Lys 364 | - | expression tag |
Ile 365 | - | expression tag |
Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 5 |
Total formula weight | 177194.5 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 218.2 | |
All* | Total formula weight | 177412.7 |