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SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

8IHB

Cryo-EM structure of HCA2-Gi complex with GSK256073

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(R)		Soluble cytochrome b562,Hydroxycarboxylic acid receptor 2polymer65874879.71UniProt (P0ABE7)
UniProt (Q8TDS4)
Pfam (PF07361)
Pfam (PF00001)		Escherichia coliCytochrome b-562,G-protein coupled receptor 109A,G-protein coupled receptor HM74A,Niacin receptor 1,Nicotinic acid receptor
2B
(A)		Guanine nucleotide-binding protein G(i) subunit alpha-1polymer35440446.01UniProt (P63096)
Pfam (PF00503)		Homo sapiens (human)Adenylate cyclase-inhibiting G alpha protein
3C
(S)		scFv16polymer24826466.51synthetic construct
4D
(B)		Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1polymer38241772.61UniProt (P62874)
Pfam (PF00400)		Mus musculus (house mouse)
5E
(C)		Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2polymer697658.91UniProt (P63213)
Pfam (PF00631)		Mus musculus (house mouse)G gamma-I
6F
(R)		2-acetamido-2-deoxy-beta-D-glucopyranosenon-polymer221.21Chemie (NAG)
7G
(R)		8-chloranyl-3-pentyl-7H-purine-2,6-dionenon-polymer256.71Chemie (OKL)
Sequence modifications
R: -104 - 0 (UniProt: P0ABE7)
PDBExternal DatabaseDetails
Met -128-initiating methionine
Lys -127-expression tag
Thr -126-expression tag
Ile -125-expression tag
Ile -124-expression tag
Ala -123-expression tag
Leu -122-expression tag
Ser -121-expression tag
Tyr -120-expression tag
Ile -119-expression tag
Phe -118-expression tag
Cys -117-expression tag
Leu -116-expression tag
Val -115-expression tag
Phe -114-expression tag
Ala -113-expression tag
Asp -112-expression tag
Tyr -111-expression tag
Lys -110-expression tag
Asp -109-expression tag
Asp -108-expression tag
Asp -107-expression tag
Asp -106-expression tag
Lys -105-expression tag
Trp -98Met 29engineered mutation
Ile -3His 124engineered mutation
Leu 1-linker
R: 2 - 363 (UniProt: Q8TDS4)
PDBExternal DatabaseDetails
Glu 364-expression tag
Asn 365-expression tag
Leu 366-expression tag
Tyr 367-expression tag
Phe 368-expression tag
Gln 369-expression tag
Gly 370-expression tag
Ser 371-expression tag
Val 372-expression tag
Phe 373-expression tag
Thr 374-expression tag
Leu 375-expression tag
Glu 376-expression tag
Asp 377-expression tag
Phe 378-expression tag
Val 379-expression tag
Gly 380-expression tag
Asp 381-expression tag
Trp 382-expression tag
Glu 383-expression tag
Gln 384-expression tag
Thr 385-expression tag
Ala 386-expression tag
Ala 387-expression tag
Tyr 388-expression tag
Asn 389-expression tag
Leu 390-expression tag
Asp 391-expression tag
Gln 392-expression tag
Val 393-expression tag
Leu 394-expression tag
Glu 395-expression tag
Gln 396-expression tag
Gly 397-expression tag
Gly 398-expression tag
Val 399-expression tag
Ser 400-expression tag
Ser 401-expression tag
Leu 402-expression tag
Leu 403-expression tag
Gln 404-expression tag
Asn 405-expression tag
Leu 406-expression tag
Ala 407-expression tag
Val 408-expression tag
Ser 409-expression tag
Val 410-expression tag
Thr 411-expression tag
Pro 412-expression tag
Ile 413-expression tag
Gln 414-expression tag
Arg 415-expression tag
Ile 416-expression tag
Val 417-expression tag
Arg 418-expression tag
Ser 419-expression tag
Gly 420-expression tag
Glu 421-expression tag
Asn 422-expression tag
Ala 423-expression tag
Leu 424-expression tag
Lys 425-expression tag
Ile 426-expression tag
Asp 427-expression tag
Ile 428-expression tag
His 429-expression tag
Val 430-expression tag
Ile 431-expression tag
Ile 432-expression tag
Pro 433-expression tag
Tyr 434-expression tag
Glu 435-expression tag
Gly 436-expression tag
Leu 437-expression tag
Ser 438-expression tag
Ala 439-expression tag
Asp 440-expression tag
Gln 441-expression tag
Met 442-expression tag
Ala 443-expression tag
Gln 444-expression tag
Ile 445-expression tag
Glu 446-expression tag
Glu 447-expression tag
Val 448-expression tag
Phe 449-expression tag
Lys 450-expression tag
Val 451-expression tag
Val 452-expression tag
Tyr 453-expression tag
Pro 454-expression tag
Val 455-expression tag
Asp 456-expression tag
Asp 457-expression tag
His 458-expression tag
His 459-expression tag
Phe 460-expression tag
Lys 461-expression tag
Val 462-expression tag
Ile 463-expression tag
Leu 464-expression tag
Pro 465-expression tag
Tyr 466-expression tag
Gly 467-expression tag
Thr 468-expression tag
Leu 469-expression tag
Val 470-expression tag
Ile 471-expression tag
Asp 472-expression tag
Gly 473-expression tag
Val 474-expression tag
Thr 475-expression tag
Pro 476-expression tag
Asn 477-expression tag
Met 478-expression tag
Leu 479-expression tag
Asn 480-expression tag
Tyr 481-expression tag
Phe 482-expression tag
Gly 483-expression tag
Arg 484-expression tag
Pro 485-expression tag
Tyr 486-expression tag
Glu 487-expression tag
Gly 488-expression tag
Ile 489-expression tag
Ala 490-expression tag
Val 491-expression tag
Phe 492-expression tag
Asp 493-expression tag
Gly 494-expression tag
Lys 495-expression tag
Lys 496-expression tag
Ile 497-expression tag
Thr 498-expression tag
Val 499-expression tag
Thr 500-expression tag
Gly 501-expression tag
Thr 502-expression tag
Leu 503-expression tag
Trp 504-expression tag
Asn 505-expression tag
Gly 506-expression tag
Asn 507-expression tag
Lys 508-expression tag
Ile 509-expression tag
Ile 510-expression tag
Asp 511-expression tag
Glu 512-expression tag
Arg 513-expression tag
Leu 514-expression tag
Ile 515-expression tag
Thr 516-expression tag
Pro 517-expression tag
Asp 518-expression tag
Gly 519-expression tag
Ser 520-expression tag
Met 521-expression tag
Leu 522-expression tag
Phe 523-expression tag
Arg 524-expression tag
Val 525-expression tag
Thr 526-expression tag
Ile 527-expression tag
Asn 528-expression tag
Ser 529-expression tag
A: 1 - 354 (UniProt: P63096)
PDBExternal DatabaseDetails
Ala 203Gly 203engineered mutation
Ala 219Thr 219conflict
Gln 288Pro 288conflict
Ser 326Ala 326engineered mutation
B: 2 - 340 (UniProt: P62874)
PDBExternal DatabaseDetails
Met -15-initiating methionine
His -14-expression tag
His -13-expression tag
His -12-expression tag
His -11-expression tag
His -10-expression tag
His -9-expression tag
His -8-expression tag
His -7-expression tag
Glu -6-expression tag
Asn -5-expression tag
Leu -4-expression tag
Tyr -3-expression tag
Phe -2-expression tag
Gln -1-expression tag
Gly 0-expression tag
Ser 1-expression tag
Gly 341-expression tag
Gly 342-expression tag
Ser 343-expression tag
Gly 344-expression tag
Gly 345-expression tag
Gly 346-expression tag
Gly 347-expression tag
Ser 348-expression tag
Gly 349-expression tag
Gly 350-expression tag
Ser 351-expression tag
Ser 352-expression tag
Ser 353-expression tag
Gly 354-expression tag
Gly 355-expression tag
Val 356-expression tag
Ser 357-expression tag
Gly 358-expression tag
Trp 359-expression tag
Arg 360-expression tag
Leu 361-expression tag
Phe 362-expression tag
Lys 363-expression tag
Lys 364-expression tag
Ile 365-expression tag
Ser 366-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains5
Total formula weight191223.7
Non-Polymers*Number of molecules2
Total formula weight477.9
All*Total formula weight191701.5
*Water molecules are not included.

243531

PDB entries from 2025-10-22

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