8IGW

Hexameric Ring Complex of Engineered V1-ATPase bound to 4 ADPs: A3(De)3_(ADP)3cat,1non-cat, Hexameric Ring Complex of Engineered V1-ATPase bound to 5 ADPs: A3(De)3_(ADP)3cat,2non-cat

This is a non-PDB format compatible entry.

Replaces:  7COS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, G, H...	V-type sodium ATPase catalytic subunit A	polymer	596	66059.5	6	UniProt (Q08636) Pfam (PF02874) Pfam (PF16886) Pfam (PF00006) In PDB	Enterococcus hirae ATCC 9790	Na(+)-translocating ATPase subunit A,V-type sodium pump catalytic subunit A
2	D, E, F, J, K...	V-type sodium ATPase subunit B	polymer	458	51017.2	6	UniProt (Q08637) Pfam (PF02874) Pfam (PF00006) In PDB	Enterococcus hirae ATCC 9790	Na(+)-translocating ATPase subunit B,V-type sodium pump subunit B
3	J, L, A, B, C...	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	9	Chemie (ADP)
4	J, L, A, B, C...	MAGNESIUM ION	non-polymer		24.3	9	Chemie (MG)

Sequence modifications

A, B, C, G, H, I: 1 - 593 (UniProt: Q08636)

PDB	External Database	Details
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

D, E, F, J, K, L: 1 - 458 (UniProt: Q08637)

PDB	External Database	Details
Gly 151	Ser 151	engineered mutation
Pro 152	Gly 152	engineered mutation
Pro 153	Ser 153	engineered mutation
Ala 155	Leu 155	engineered mutation
Gly 156	Pro 156	engineered mutation
Lys 157	His 157	engineered mutation
Ser 158	Lys 158	engineered mutation
Ala 159	Glu 159	engineered mutation
Glu 248	Thr 248	engineered mutation
Ser 339	Gln 339	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	702460.6
Non-Polymers*	Number of molecules	18
	Total formula weight	4063.6
All*	Total formula weight	706524.2

*Water molecules are not included.