8IGS
Cryo-EM structure of RNAP-promoter open complex at lambda promoter PRE
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (H) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 1 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli (strain K12) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | B (I) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli (strain K12) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | C (J) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli (strain K12) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | D (K) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli (strain K12) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | E (L) | RNA polymerase sigma factor RpoD | polymer | 613 | 70352.2 | 1 | UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Escherichia coli (strain K12) | Sigma-70 |
| 6 | F (N) | non-template strand DNA | polymer | 85 | 26185.8 | 1 | Escherichia coli (strain K12) | ||
| 7 | G (T) | template strand DNA | polymer | 85 | 26235.9 | 1 | Escherichia coli (strain K12) | ||
| 8 | H (J) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 9 | I, J (J) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 475769.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 155.1 | |
| All* | Total formula weight | 475924.9 |
