8IFL
Cryo-EM structure of tetrameric SPARTA gRNA-ssDNA target complex in state 1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E, I, M (A, F, J, N) | TIR domain-containing protein | polymer | 450 | 53256.7 | 4 | UniProt (A0A1I7NFG5) Pfam (PF13676) | Thermoflavifilum thermophilum | CrtTIR-APAZ |
| 2 | B, F, J, N (B, G, K, O) | Piwi domain-containing protein | polymer | 507 | 58304.8 | 4 | UniProt (A0A1I7NFD7) | Thermoflavifilum thermophilum | CrtAgo |
| 3 | C, G, K, O (C, H, L, P) | guide RNA | polymer | 21 | 6659.0 | 4 | Escherichia coli 'BL21-Gold(DE3)pLysS AG' | ||
| 4 | D, H, L, P (D, I, M, Q) | target ssDNA | polymer | 25 | 7681.0 | 4 | Escherichia coli 'BL21-Gold(DE3)pLysS AG' | ||
| 5 | Q, R, S, T (B, H, L, P) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 16 |
| Total formula weight | 503606.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 97.2 | |
| All* | Total formula weight | 503703.5 |
