8IFL

Cryo-EM structure of tetrameric SPARTA gRNA-ssDNA target complex in state 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F, J, N	TIR domain-containing protein	polymer	450	53256.7	4	UniProt (A0A1I7NFG5) Pfam (PF13676) In PDB	Thermoflavifilum thermophilum	CrtTIR-APAZ
2	B, G, K, O	Piwi domain-containing protein	polymer	507	58304.8	4	UniProt (A0A1I7NFD7) In PDB	Thermoflavifilum thermophilum	CrtAgo
3	C, H, L, P	guide RNA	polymer	21	6659.0	4		Escherichia coli 'BL21-Gold(DE3)pLysS AG'
4	D, I, M, Q	target ssDNA	polymer	25	7681.0	4		Escherichia coli 'BL21-Gold(DE3)pLysS AG'
5	B, H, L, P	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	503606.3
Non-Polymers*	Number of molecules	4
	Total formula weight	97.2
All*	Total formula weight	503703.5

*Water molecules are not included.