8IEG

Bre1(mRBD-RING)/Rad6-Ub/nucleosome complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (K, E)	Histone H3.1	polymer	98	11562.6	2	UniProt (P68431) Pfam (PF00125)	Homo sapiens (human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B, F (L, F)	Histone H4	polymer	80	9123.7	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	109	11936.9	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D (D)	Histone H2B type 1-K	polymer	94	10452.0	1	UniProt (O60814) Pfam (PF00125)	Homo sapiens (human)	H2B K,HIRA-interacting protein 1
5	H (H)	Histone H2B type 1-K	polymer	94	10478.0	1	UniProt (O60814) Pfam (PF00125)	Homo sapiens (human)	H2B K,HIRA-interacting protein 1
6	I (I)	DNA (147-MER)	polymer	147	45604.0	1		Homo sapiens (human)
7	J (J)	DNA (147-MER)	polymer	147	45145.8	1		Homo sapiens (human)
8	K, L (A, B)	E3 ubiquitin-protein ligase BRE1	polymer	64	7357.7	2	UniProt (Q07457) Pfam (PF00097)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Brefeldin A-sensitivity protein 1,RING-type E3 ubiquitin transferase BRE1
9	M (R)	Ubiquitin-conjugating enzyme E2 2	polymer	148	16973.2	1	UniProt (P06104) Pfam (PF00179)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	E2 ubiquitin-conjugating enzyme 2,Radiation sensitivity protein 6,Ubiquitin carrier protein UBC2,Ubiquitin-protein ligase UBC2
10	N, O, P, Q (A, B)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

D: 31 - 124 (UniProt: O60814)

PDB	External Database	Details
Cys 120	Lys 121	conflict

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	208614.8
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	208876.4

*Water molecules are not included.