8IBT
Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis E318S mutant in complex with lacto-N-tetraose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Beta-galactosidase | polymer | 702 | 78764.8 | 2 | UniProt (B7GUD7) Pfam (PF02449) Pfam (PF08532) Pfam (PF08533) UniProt (by SIFTS) (Q93GI5) | Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12) | |
| 2 | C, D (C, D) | beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose | branched | 707.6 | 2 | In PDB GlyTouCan (G00049MO) | |||
| 3 | E, F (A, B) | water | water | 18.0 | 650 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 691 (UniProt: B7GUD7)
| PDB | External Database | Details |
|---|---|---|
| Ser 318 | Glu 318 | engineered mutation |
| Ala 692 | - | expression tag |
| Ala 693 | - | expression tag |
| Ala 694 | - | expression tag |
| Leu 695 | - | expression tag |
| Glu 696 | - | expression tag |
| His 697 | - | expression tag |
| His 698 | - | expression tag |
| His 699 | - | expression tag |
| His 700 | - | expression tag |
| His 701 | - | expression tag |
| His 702 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 157529.6 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1415.3 | |
| All* | Total formula weight | 158944.9 |
