8IBT

Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis E318S mutant in complex with lacto-N-tetraose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-galactosidase	polymer	702	78764.8	2	UniProt (B7GUD7) Pfam (PF02449) Pfam (PF08532) Pfam (PF08533) UniProt (by SIFTS) (Q93GI5) In PDB	Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
2	C, D	beta-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose-(1-4)-beta-D-glucopyranose	branched		707.6	2	In PDB GlyTouCan (G00049MO)
3		water	water		18.0	650	Chemie (HOH)

Sequence modifications

A, B: 1 - 691 (UniProt: B7GUD7)

PDB	External Database	Details
Ser 318	Glu 318	engineered mutation
Ala 692	-	expression tag
Ala 693	-	expression tag
Ala 694	-	expression tag
Leu 695	-	expression tag
Glu 696	-	expression tag
His 697	-	expression tag
His 698	-	expression tag
His 699	-	expression tag
His 700	-	expression tag
His 701	-	expression tag
His 702	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	157529.6
Branched	Number of molecules	2
	Total formula weight	1415.3
All*	Total formula weight	158944.9

*Water molecules are not included.