8IBS

Crystal structure of GH42 beta-galactosidase BiBga42A from Bifidobacterium longum subspecies infantis E160A/E318A mutant in complex with galactose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Beta-galactosidase	polymer	702	78690.8	6	UniProt (B7GUD7) Pfam (PF02449) Pfam (PF08532) Pfam (PF08533) UniProt (by SIFTS) (Q93GI5)	Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
2	G, H, I, J, K... (A, B, C, D, E...)	alpha-D-galactopyranose	non-polymer		180.2	6	Chemie (GLA)
3	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	2870	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 691 (UniProt: B7GUD7)

PDB	External Database	Details
Ala 160	Glu 160	engineered mutation
Ala 318	Glu 318	engineered mutation
Ala 692	-	expression tag
Ala 693	-	expression tag
Ala 694	-	expression tag
Leu 695	-	expression tag
Glu 696	-	expression tag
His 697	-	expression tag
His 698	-	expression tag
His 699	-	expression tag
His 700	-	expression tag
His 701	-	expression tag
His 702	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	472144.7
Non-Polymers*	Number of molecules	6
	Total formula weight	1080.9
All*	Total formula weight	473225.7

*Water molecules are not included.