8IBK
Crystal structure of Bacillus sp. AHU2216 GH13_31 Alpha-glucosidase E256Q/N258G in complex with maltotriose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Alpha-glucosidase | polymer | 563 | 65942.6 | 1 | UniProt (A0A2Z5WH92) Pfam (PF00128) Pfam (PF16657) | Bacillus sp. (in: firmicutes) | |
| 2 | B (C) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 504.4 | 1 | In PDB GlyTouCan (G96370VA) | |||
| 3 | C, D (A) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 4 | E (A) | water | water | 18.0 | 564 | Chemie (HOH) |
Sequence modifications
A: 1 - 555 (UniProt: A0A2Z5WH92)
| PDB | External Database | Details |
|---|---|---|
| Gln 256 | Glu 256 | engineered mutation |
| Gly 258 | Asn 258 | engineered mutation |
| Leu 556 | - | expression tag |
| Glu 557 | - | expression tag |
| His 558 | - | expression tag |
| His 559 | - | expression tag |
| His 560 | - | expression tag |
| His 561 | - | expression tag |
| His 562 | - | expression tag |
| His 563 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 65942.6 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 504.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 80.2 | |
| All* | Total formula weight | 66527.2 |
