8IBK

Crystal structure of Bacillus sp. AHU2216 GH13_31 Alpha-glucosidase E256Q/N258G in complex with maltotriose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Alpha-glucosidase	polymer	563	65942.6	1	UniProt (A0A2Z5WH92)	Bacillus sp. (in: firmicutes)
2	B (C)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		504.4	1	In PDB GlyTouCan (G96370VA)
3	C, D (A)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	E (A)	water	water		18.0	564	Chemie (HOH)

Sequence modifications

A: 1 - 555 (UniProt: A0A2Z5WH92)

PDB	External Database	Details
Gln 256	Glu 256	engineered mutation
Gly 258	Asn 258	engineered mutation
Leu 556	-	expression tag
Glu 557	-	expression tag
His 558	-	expression tag
His 559	-	expression tag
His 560	-	expression tag
His 561	-	expression tag
His 562	-	expression tag
His 563	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	65942.6
Branched	Number of molecules	1
	Total formula weight	504.4
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	66527.2

*Water molecules are not included.