8IAT

Crystal structure of Streptococcus pneumoniae pyruvate kinase in complex with oxalate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Pyruvate kinase	polymer	521	56993.6	4	UniProt (Q8DQ84) Pfam (PF00224) Pfam (PF02887) In PDB	Streptococcus pneumoniae R6
2	A, B, C, D	OXALATE ION	non-polymer		88.0	8	Chemie (OXL)
3	A, B, C, D	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	A, B, C, D	POTASSIUM ION	non-polymer		39.1	4	Chemie (K)
5	A	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
6	A, C	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
7	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
8		water	water		18.0	1019	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 501 (UniProt: Q8DQ84)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	227974.4
Non-Polymers*	Number of molecules	20
	Total formula weight	1544.4
All*	Total formula weight	229518.8

*Water molecules are not included.