8IAJ

Cryo-EM structure of the yeast SPT-ORM2 (ORM2-S3A) complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	chimera of Long chain base biosynthesis protein 1 and Serine palmitoyltransferase 1	polymer	534	59354.7	2	UniProt (Q94IB8) UniProt (P25045) Pfam (PF00155) In PDB	Arabidopsis thaliana	AtLCB1,Protein EMBRYO DEFECTIVE 2779,Protein FUMONISIN B1 RESISTANT 11,SPT 1,SPT1,Long chain base biosynthesis protein 1
2	B, F	Serine palmitoyltransferase 2	polymer	561	63189.7	2	UniProt (P40970) Pfam (PF00155) In PDB	Saccharomyces cerevisiae (baker's yeast)	SPT 2,Long chain base biosynthesis protein 2
3	D, H	Protein ORM2	polymer	216	24830.6	2	UniProt (Q06144) Pfam (PF04061) In PDB	Saccharomyces cerevisiae S288C
4	C, G	Serine palmitoyltransferase-regulating protein TSC3	polymer	80	9590.2	2	UniProt (Q3E790) In PDB	Saccharomyces cerevisiae (baker's yeast)	Temperature-sensitive CSG2-mutant suppressor protein 3
5	B, F	PYRIDOXAL-5'-PHOSPHATE	non-polymer		247.1	2	Chemie (PLP)
6	B, F	N-[(2S,3R,4E)-1,3-dihydroxyoctadec-4-en-2-yl]tetracosanamide	non-polymer		650.1	2	Chemie (Z1T)

Sequence modifications

A, E: 25 - 101 (UniProt: Q94IB8)
A, E: 102 - 558 (UniProt: P25045)
D, H: 1 - 216 (UniProt: Q06144)

PDB	External Database	Details
Ala 46	Ser 46	engineered mutation
Ala 47	Ser 47	engineered mutation
Ala 48	Ser 48	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	313930.4
Non-Polymers*	Number of molecules	4
	Total formula weight	1794.5
All*	Total formula weight	315724.9

*Water molecules are not included.