8IA2

Structure of C5a bound human C5aR1 in complex with Go (Composite map)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	C5a anaphylatoxin chemotactic receptor 1	polymer	406	45273.9	1	UniProt (P21730) Pfam (PF00001)	Homo sapiens (human)	C5a anaphylatoxin chemotactic receptor,C5a-R,C5aR
2	B (D)	C5a anaphylatoxin	polymer	74	8288.7	1	UniProt (P01031) Pfam (PF01821)	Homo sapiens (human)
3	C (B)	Guanine nucleotide-binding protein G(o) subunit alpha	polymer	250	28193.9	1	UniProt (P09471) Pfam (PF00503)	Homo sapiens (human)
4	D (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
6	F (H)	Antibody fragment - ScFv16	polymer	248	26466.5	1		Mus musculus

Sequence modifications

A: 2 - 350 (UniProt: P21730)

PDB	External Database	Details
Met -55	-	initiating methionine
Gly -54	-	expression tag
Lys -53	-	expression tag
Thr -52	-	expression tag
Ile -51	-	expression tag
Ile -50	-	expression tag
Ala -49	-	expression tag
Leu -48	-	expression tag
Ser -47	-	expression tag
Tyr -46	-	expression tag
Ile -45	-	expression tag
Phe -44	-	expression tag
Cys -43	-	expression tag
Leu -42	-	expression tag
Val -41	-	expression tag
Phe -40	-	expression tag
Ala -39	-	expression tag
Asp -38	-	expression tag
Tyr -37	-	expression tag
Lys -36	-	expression tag
Asp -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Ala -31	-	expression tag
Ala -30	-	expression tag
Asn -29	-	expression tag
Phe -28	-	expression tag
Thr -27	-	expression tag
Pro -26	-	expression tag
Val -25	-	expression tag
Asn -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
Gly -20	-	expression tag
Asn -19	-	expression tag
Gln -18	-	expression tag
Ser -17	-	expression tag
Val -16	-	expression tag
Arg -15	-	expression tag
Leu -14	-	expression tag
Val -13	-	expression tag
Thr -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Leu -8	-	expression tag
Glu -7	-	expression tag
Val -6	-	expression tag
Leu -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag

B: 4 - 173 (UniProt: P09471)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Glu -3	-	expression tag
Asn -2	-	expression tag
Leu -1	-	expression tag
Tyr 0	-	expression tag
Phe 1	-	expression tag
Gln 2	-	expression tag
Gly 3	-	expression tag
Asp 42	Gly 42	engineered mutation
Asn 43	Glu 43	engineered mutation

B: 182 - 354 (UniProt: P09471)

PDB	External Database	Details
Gly 174	-	linker
Gly 175	-	linker
Ser 176	-	linker
Gly 177	-	linker
Gly 178	-	linker
Ser 179	-	linker
Gly 180	-	linker
Gly 181	-	linker
Asp 227	Ala 227	engineered mutation
Asp 230	Gly 230	engineered mutation
Ala 332	Ile 332	engineered mutation
Ile 335	Val 335	engineered mutation

C: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	initiating methionine
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	154618.2
All*	Total formula weight	154618.2

*Water molecules are not included.